Turn-on fluorescent probe for sensing exogenous and endogenous hypochlorous acid in living cells, zebrafishes and mice

被引：36
作者：
Gan, Yabing [1 ]
Yin, Guoxing [1 ]
Zhang, Xiaming [1 ]
Zhou, Li [2 ,3 ]
Zhang, Yibao [4 ]
Li, Haitao [1 ]
Yin, Peng [1 ]
机构：
[1] Hunan Normal Univ, Coll Chem & Chem Engn, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ, Changsha 410081, Peoples R China
[2] Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Dept Radiat Oncol, Chengdu 610041, Peoples R China
[3] Sichuan Univ, West China Hosp, Canc Ctr, Chengdu 610041, Peoples R China
[4] Peking Univ, Dept Radiat Oncol, Key Lab Carcinogenesis & Translat Res, Minist Educ Beijing,Canc Hosp & Inst, Beijing 100142, Peoples R China
来源：
TALANTA | 2021年 / 225卷
基金：
中国国家自然科学基金;
关键词：
Fluorescent probe; Hypochlorous acid; Large Stokes shift; High quantum yield; RATIOMETRIC DETECTION; SI-RHODAMINE; LIVE CELLS; MITOCHONDRIA; MYELOPEROXIDASE; HCLO; RED; ROS;
D O I：
10.1016/j.talanta.2020.122030
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Hypochlorous acid (HOCl), belonging to biologically significant reactive oxygen species (ROS), plays crucial roles in many biological and pathological processes. It is of great value to explore fluorescent probes for the image of hypochlorous acid in various biological environments. We herein reported a novel fluorescent probe HN-ClO for monitoring HOCl with moderate water-solubility, good photostability, high fluorescence quantum yield and large Stokes shift. This probe exhibited excellent selectivity and high sensitivity to sense HOCl. Furthermore, probe HN-ClO was successfully applied to monitor endogenous and exogenous HOCl in living cells, zebrafishes and mice, and possessed the potential to further explore the physiological and pathological roles of hypochlorous acid in biological systems.
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页数：8
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