Engineered Skin Substitute Regenerates the Skin with Hair Follicle Formation

被引：8

作者：

Wang, Jinmei ^{[1
,2
,3
]}

Wang, Xiaoxiao ^{[2
,4
,5
]}

Xie, Jundong ^{[2
,3
,4
]}

Yao, Bin ^{[2
,3
,4
]}

Mo, Miaohua ^{[2
,3
,4
]}

Ma, Danjun ^{[1
]}

Huang, Chen ^{[6
]}

Xu, Renhe ^{[7
]}

Fu, Xiaobing ^{[8
,9
]}

Tredget, Edward E. ^{[10
]}

Wu, Yaojiong ^{[2
,3
,4
]}

机构：

[1] Dongguan Univ Technol, Sch Mech Engn, 1 Daxue Rd, Dongguan 523830, Peoples R China

[2] Tsinghua Univ, Shenzhen Int Grad Sch, iBHE, Shenzhen 518055, Peoples R China

[3] Tsinghua Univ, Shenzhen Int Grad Sch, State Key Lab Chem Oncogen, Shenzhen 518055, Peoples R China

[4] Tsinghua Univ, Tsinghua Berkeley Shenzhen Inst TBSI, Shenzhen 518055, Peoples R China

[5] Southern Univ Sci & Technol, Shenzhen Key Lab Cell Microenvironm, Guangdong Prov Key Lab Cell Microenvironm & Dis R, Dept Biol, Shenzhen 518055, Peoples R China

[6] Xi An Jiao Tong Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Cell Biol & Genet, Xian 710061, Peoples R China

[7] Univ Macau, Fac Hlth Sci, Macau 999078, Peoples R China

[8] Chinese Peoples Liberat Army Gen Hosp, Inst Basic Med Sci, Wound Healing & Cell Biol Lab, Beijing 100088, Peoples R China

[9] Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Stem Cell & Tissue Regenerat Lab, Beijing 100084, Peoples R China

[10] Univ Alberta, Dept Surg, Wound Healing Res Grp, Edmonton, AB T6G 2B7, Canada

来源：

BIOMEDICINES | 2021年 / 9卷 / 04期

基金：

中国博士后科学基金; 国家重点研发计划;

关键词：

skin derived precursors; hair follicle neogenesis; engineered skin substitute;

D O I：

10.3390/biomedicines9040400

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Currently, engineered skin substitutes (ESS) are unable to regenerate cutaneous appendages. Recent studies have shown that skin-derived precursors (SKPs), which are extensively available, have the potential to induce hair follicle neogenesis. Here, we demonstrate that ESS consisting of culture-expanded SKPs and epidermal stem cells (Epi-SCs) reconstitute the skin with hair follicle regeneration after grafting into nude mice. SKPs seeded in a C-GAG matrix proliferated and expressed higher levels of hair induction signature genes-such as Akp2, Sox2, CD133 and Bmp6-compared to dermal fibroblasts. Moreover, when ESS prepared by seeding a mixture of culture-expanded murine SKPs and human adult Epi-SCs into a C-GAG matrix was grafted into full-thickness skin wounds in nude mice, black hairs were generated within 3 weeks. Immunofluorescence analysis showed that the SKPs were localized to the dermal papillae of the newly-formed hair follicle. Our results indicate that SKPs can serve as the hair-inductive cells in ESS to furnish it with hair genesis potential

引用

页数：15

共 43 条

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