A colorimetric strategy based on a water-soluble conjugated polymer for sensing pH-driven conformational conversion of DNA i-motif structure

被引：22

作者：

Wang, Lihua ^{[1
]}

Liu, Xingfen ^{[2
]}

Yang, Qing ^{[1
]}

Fan, Quli ^{[2
]}

Song, Shiping ^{[1
]}

Fan, Chunhai ^{[1
]}

Huang, Wei ^{[2
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

[2] Nanjing Univ Posts & Telecommun, Nanjing 210003, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2010年 / 25卷 / 07期

关键词：

Colorimetric biosensor; Water-soluble conjugated polymer; DNA i-motif structure; Conformational conversion; NUCLEIC-ACID PROBES; G-QUADRUPLEX; GOLD NANOPARTICLES; CHEMICAL SENSORS; OPTICAL-DETECTION; ENERGY-TRANSFER; DUPLEX; TRANSITION; PROTEINS; HYBRID;

D O I：

10.1016/j.bios.2009.12.016

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Using a water-soluble conjugated polymer (CP) as a sensing probe, we developed a rapid colorimetric detection strategy for pH-driven conformational conversion of DNA i-motif structure. Two sensing configurations were designed: one used CP only to detect the conversion between i-motif and random-coiled state of a C-rich single-strand DNA, the other used CP and a complementary single-strand DNA to investigate the conversion of duplex to i-motif equilibrium. All the conversions would lead to color change observed directly with naked eyes within a few minutes. The limitation of detection (LOD) is as low as 40 nM. More importantly, reversible conformational conversions by adjusting the pH of the system could also be detected. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1838 / 1842

页数：5

共 38 条

[1] HUMAN TELOMERIC C-STRAND TETRAPLEXES [J].

AHMED, S ;

KINTANAR, A ;

HENDERSON, E .

NATURE STRUCTURAL BIOLOGY, 1994, 1 (02) :83-88

[2] Raman signature of the four-stranded intercalated cytosine motif in crystal and solution structures of DNA deoxycytidylates d(CCCT) and d(C-8) [J].

Benevides, JM ;

Kang, C ;

Thomas, GJ .

BIOCHEMISTRY, 1996, 35 (18) :5747-5755

[3] Highly sensitive biological and chemical sensors based on reversible fluorescence quenching in a conjugated polymer [J].

Chen, LH ;

McBranch, DW ;

Wang, HL ;

Helgeson, R ;

Wudl, F ;

Whitten, DG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (22) :12287-12292

[4] Hybridization of PNA to structured DNA targets: Quadruplex invasion and the overhang effect [J].

Datta, B ;

Armitage, BA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (39) :9612-9619

[5] DUPLEX TO QUADRUPLEX EQUILIBRIUM OF THE SELF-COMPLEMENTARY OLIGONUCLEOTIDE D(GGGGCCCC) [J].

DENG, H ;

BRAUNLIN, WH .

BIOPOLYMERS, 1995, 35 (06) :677-681

[6] Fluorescent polymeric transducer for the rapid, simple, and specific detection of nucleic acids at the zeptomole level [J].

Doré, K ;

Dubus, S ;

Ho, HA ;

Lévesque, I ;

Brunette, M ;

Corbeil, G ;

Boissinot, M ;

Boivin, G ;

Bergeron, MG ;

Boudreau, D ;

Leclerc, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (13) :4240-4244

[7] Biosensors based on binding-modulated donor-acceptor distances [J].

Fan, CH ;

Plaxco, KW ;

Heeger, AJ .

TRENDS IN BIOTECHNOLOGY, 2005, 23 (04) :186-192

[8] High-efficiency fluorescence quenching of conjugated polymers by proteins [J].

Fan, CH ;

Plaxco, KW ;

Heeger, AJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (20) :5642-5643

[9] Beyond superquenching: Hyper-efficient energy transfer from conjugated polymers to gold nanoparticles [J].

Fan, CH ;

Wang, S ;

Hong, JW ;

Bazan, GC ;

Plaxco, KW ;

Heeger, AJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (11) :6297-6301

[10] SNP detection using peptide nucleic acid probes and conjugated polymers: Applications in neurodegenerative disease identification [J].

Gaylord, BS ;

Massie, MR ;

Feinstein, SC ;

Bazan, GC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (01) :34-39

← 1 2 3 4 →