Design and synthesis of a minimal bacterial genome

被引：903

作者：

Hutchison, Clyde A., III ^{[1
]}

Chuang, Ray-Yuan ^{[1
,5
]}

Noskov, Vladimir N. ^{[1
]}

Assad-Garcia, Nacyra ^{[1
]}

Deerinck, Thomas J. ^{[2
]}

Ellisman, Mark H. ^{[2
]}

Gill, John ^{[3
]}

Kannan, Krishna ^{[3
]}

Karas, Bogumil J. ^{[1
]}

Ma, Li ^{[1
]}

Pelletier, James F. ^{[4
,6
]}

Qi, Zhi-Qing ^{[3
]}

Richter, R. Alexander ^{[1
]}

Strychalski, Elizabeth A. ^{[4
]}

Sun, Lijie ^{[1
,7
]}

Suzuki, Yo ^{[1
]}

Tsvetanova, Billyana ^{[3
]}

Wise, Kim S. ^{[1
]}

Smith, Hamilton O. ^{[1
,3
]}

Glass, John I. ^{[1
]}

Merryman, Chuck ^{[1
]}

Gibson, Daniel G. ^{[1
,3
]}

Venter, J. Craig ^{[1
,3
]}

机构：

[1] J Craig Venter Inst, La Jolla, CA 92037 USA

[2] Univ Calif San Diego, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92037 USA

[3] Synthet Genom, La Jolla, CA 92037 USA

[4] NIST, Gaithersburg, MD 20899 USA

[5] Amer Type Culture Collect, 10801 Univ Blvd, Manassas, VA 20110 USA

[6] MIT, Ctr Bits & Atoms, Room E15-401,20 Ames St, Cambridge, MA 02139 USA

[7] CBRITE, 11575 Sorrento Valley Rd,Suite 204, San Diego, CA 92121 USA

来源：

SCIENCE | 2016年 / 351卷 / 6280期

关键词：

ESSENTIAL GENES; MYCOPLASMA; SEPF;

D O I：

10.1126/science.aad6253

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We used whole-genome design and complete chemical synthesis to minimize the 1079-kilobase pair synthetic genome of Mycoplasma mycoides JCVI-syn1.0. An initial design, based on collective knowledge of molecular biology combined with limited transposon mutagenesis data, failed to produce a viable cell. Improved transposon mutagenesis methods revealed a class of quasi-essential genes that are needed for robust growth, explaining the failure of our initial design. Three cycles of design, synthesis, and testing, with retention of quasi-essential genes, produced JCVI-syn3.0 (531 kilobase pairs, 473 genes), which has a genome smaller than that of any autonomously replicating cell found in nature. JCVI-syn3.0 retains almost all genes involved in the synthesis and processing of macromolecules. Unexpectedly, it also contains 149 genes with unknown biological functions. JCVI-syn3.0 is a versatile platform for investigating the core functions of life and for exploring whole-genome design.

引用

页数：11

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