Mouse redox histology using genetically encoded probes

被引：53

作者：

Fujikawa, Yuuta ^{[1
,11
,12
]}

Roma, Leticia P. ^{[1
]}

Sobotta, Mirko C. ^{[1
]}

Rose, Adam J. ^{[2
,3
]}

Diaz, Mauricio Berriel ^{[2
,3
,4
,5
]}

Locatelli, Giuseppe ^{[6
]}

Breckwoldt, Michael O. ^{[6
,7
]}

Misgeld, Thomas ^{[7
,8
,9
]}

Kerschensteiner, Martin ^{[6
,8
]}

Herzig, Stephan ^{[2
,3
,4
,5
]}

Mueller-Decker, Karin ^{[10
]}

Dick, Tobias P. ^{[1
]}

机构：

[1] DKFZ ZMBH German Canc Res Ctr Ctr Mol Biol Univ H, Div Redox Regulat, DKFZ, Neuenheimer Feld 280, D-69120 Heidelberg, Germany

[2] Heidelberg Univ, DKFZ ZMBH Alliance, DKFZ Heidelberg, Ctr Mol Biol ZMBH,Joint Div Mol Metab Control, D-69120 Heidelberg, Germany

[3] Heidelberg Univ, Univ Hosp, D-69120 Heidelberg, Germany

[4] Univ Heidelberg Hosp, Helmholtz Ctr Munich, Inst Diabet & Canc, D-85764 Neuherberg, Germany

[5] Univ Heidelberg Hosp, Helmholtz Ctr Munich, Joint Heidelberg IDC Translat Diabet Program, D-85764 Neuherberg, Germany

[6] Univ Munich, Inst Clin Neuroimmunol, Marchioninistr 15, D-81377 Munich, Germany

[7] Tech Univ Munich, Inst Neuronal Cell Biol, D-80802 Munich, Germany

[8] Munich Cluster Syst Neurol SyNergy, D-81377 Munich, Germany

[9] German Ctr Neurodegenerat Dis DZNE, D-81377 Munich, Germany

[10] DKFZ, Core Facil Tumor Models, D-69120 Heidelberg, Germany

[11] Tokyo Univ Pharm & Life Sci, Sch Life Sci, 1432-1 Horinouchi, Hachioji, Tokyo 1920392, Japan

[12] Heidelberg Univ, Dept Neuroradiol, Neuenheimer Feld 400, D-69120 Heidelberg, Germany

来源：

SCIENCE SIGNALING | 2016年 / 9卷 / 419期

基金：

欧洲研究理事会; 巴西圣保罗研究基金会; 日本学术振兴会;

关键词：

HYDROGEN-PEROXIDE; OXIDATIVE STRESS; CELLS; H2O2;

D O I：

10.1126/scisignal.aad3895

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mapping the in vivo distribution of endogenous oxidants in animal tissues is of substantial biomedical interest. Numerous health-related factors, including diet, physical activity, infection, aging, toxins, or pharmacological intervention, may cause redox changes. Tools are needed to pinpoint redox state changes to particular organs, tissues, cell types, and subcellular organelles. We describe a procedure that preserves the in vivo redox state of genetically encoded redox biosensors within histological tissue sections, thus providing "redox maps" for any tissue and comparison of interest. We demonstrate the utility of the technique by visualizing endogenous redox differences and changes in the context of tumor growth, inflammation, embryonic development, and nutrient starvation.

引用

页数：10

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