Establishment of a Novel Temozolomide Resistant Subline of Glioblastoma Multiforme Cells and Comparative Transcriptome Analysis With Parental Cells

被引：5

作者：

Ha, Hai-Anh ^{[1
,3
]}

Yang, Jai-Sing ^{[4
]}

Tsai, Fuu-Jen ^{[2
,5
,6
]}

Li, Chang-Wei ^{[7
]}

Cheng, Yih-Dih ^{[1
]}

Li, Jianyong ^{[8
]}

Hour, Mann-Jen ^{[1
]}

Chiu, Yu-Jen ^{[9
,10
,11
]}

机构：

[1] China Med Univ, Sch Pharm, 91 Hsueh Shih Rd, Taichung 40402, Taiwan

[2] China Med Univ, Sch Chinese Med, Taichung, Taiwan

[3] Duy Tan Univ, Fac Pharm, Da Nang, Vietnam

[4] China Med Univ, China Med Univ Hosp, Dept Med Res, Taichung, Taiwan

[5] China Med Univ Hosp, Dept Med Res, Ctr Human Genet, Taichung, Taiwan

[6] China Med Univ Hosp, Dept Med Genet, Taichung, Taiwan

[7] Natl Yang Ming Chiao Tung Univ, Dept Biol Sci & Technol, Hsinchu, Taiwan

[8] Third Mil Med Univ, Army Med Univ, Chongqing, Peoples R China

[9] Taipei Vet Gen Hosp, Div Plast & Reconstruct Surg, Dept Surg, 201,Sect 2,Shipai Rd, Taipei 11217, Taiwan

[10] Natl Yang Ming Chiao Tung Univ, Sch Med, Dept Surg, Taipei, Taiwan

[11] Natl Yang Ming Chiao Tung Univ, Inst Clin Med, Taipei, Taiwan

来源：

ANTICANCER RESEARCH | 2021年 / 41卷 / 05期

关键词：

Glioblastoma multiforme; temozolomide (TMZ); TMZ resistance; GBM-8401; TMZR; MGMT-mediated DNA repair; mismatch repair; LINE U251; APOPTOSIS; OVERCOME;

D O I：

10.21873/anticanres.15008

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background/Aim: Glioblastoma multiforme (GBM) is a lethal disease with a high rate of chemoresistance to temozolomide (TMZ). The aim of the study was to establish a TMZ-resistant subline from the GBM-8401 cell line to determine the mechanisms of resistance and identify novel effective therapeutics for TMZ-resistant GBM. Materials and Methods: Comparative transcriptome analysis of GBM-8401/TMZR cells and the parental line was performed using Ion Torrent sequencing. Differentially expressed genes (DEGs) between the GBM-8401/TMZR and GBM-8401 cell lines were analyzed. Results: Transcriptomic profiling of GBM-8401/TMZR cells revealed DEGs involved in the retinoblastoma (RB) signaling, DNA damage response (DDR) pathway, and DNA repair mechanisms. Conclusion: In vitro and in vivo cell -based GBM models should be used in further biomedical studies to investigate the underlying mechanisms of TMZ-resistant GBM.

引用

页码：2333 / 2347

页数：15

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