Life cycle assessment of fish oil substitute produced by microalgae using food waste

Fish oil has been used in conventional aquaculture for decades, despite the known links between increasing global demand for fish and depletion of natural resources and vital ecosystems (FAO, 2020, 2019). Alternative feed ingredients, including algae oil rich in docosahexaenoic acid (DHA), has therefore been increasingly used to substitute traditional fish oil. Heterotrophic algae cultivation in bioreactors can be supported by a primary carbon feedstock recovered from food waste, a solution that could reduce environmental impacts and support the transition towards circular food systems. This study used life cycle assessment to quantify environmental impact of DHA produced by the heterotrophic algae Crypthecodinium cohnii, using short-chain carboxylic acids derived from dark fermentation of food waste. The future potential of DHA from algae was evaluated by comparing the environmental impact to that of DHA from Peruvian anchovy oil. With respect to global warming, terrestrial acidification, freshwater eutrophication and land use, algae oil inferred-52 ton CO(2)eq, 3.5 ton SO(2)eq,-94 kg Peq, 2700 m(2) eq, respectively per ton DHA. In comparison, the impact per ton DHA from fish oil was-15 ton CO(2)eq, 3.9 ton SO(2)eq,-97 kg Peq and 3200 m(2) eq. Furthermore, algae oil showed lower climate impact compared to canola and linseed oil. By including Ecosystem damage as indicator for ecosystem quality at endpoint level, the important aspect of biodiversity impact was accounted for. Although the method primarily accounts for indirect effects on biodiversity, DHA from algae oil showed lower Ecosystem damage compared to fish oil even when future energy development, optimized production, increased energy demand and effects on biotic resources were considered via sensitivity analyses. As the results suggest, algae oil holds a promising potential for increased sustainability within aquaculture, provided that continued development and optimization of this emerging technology is enabled through active decision-making and purposeful investments. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of Institution of Chemical Engineers.