Population Genomics of the Neotropical Brown Stink Bug, Euschistus heros: The Most Important Emerging Insect Pest to Soybean in Brazil

被引:18
作者
Zucchi, Maria, I [1 ,2 ]
Cordeiro, Erick M. G. [3 ,8 ]
Wu, Xing [4 ]
Lamana, Leticia Marise [5 ]
Brown, Patrick J. [4 ]
Manjunatha, Shilpa [4 ]
Gomes Viana, Joao Paulo [4 ]
Omoto, Celso [3 ]
Pinheiro, Jose B. [6 ]
Clough, Steven J. [4 ,7 ]
机构
[1] Univ Estadual Campinas, Inst Biol, Campinas, SP, Brazil
[2] Agencia Paulista Tecnol Agronegocios, Polo Reg Ctr Sul, Piracicaba, Brazil
[3] Univ Sao Paulo, Dept Entomol, ESALQ, Piracicaba, Brazil
[4] Univ Illinois, Dept Crop Sci, Urbana, IL USA
[5] Univ Estadual Ponta Grossa, Dept Fitotecnia & Fitossanidade, Ponta Grossa, Parana, Brazil
[6] Univ Sao Paulo, Dept Genet, ESALQ, Piracicaba, Brazil
[7] ARS, USDA, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL USA
[8] Univ Exeter, Penryn Campus, Penryn TR10 9FE, Cornwall, England
基金
巴西圣保罗研究基金会;
关键词
soybean pest; genotyping by sequencing; population genomics; single-nucleotide polymorphism marker; Euschistus heros; Neotropical brown stink bug; HEMIPTERA HETEROPTERA-PENTATOMIDAE; SECONDARY PESTS; BT-COTTON; R PACKAGE; EVOLUTION; SELECTION; LOCI; PLANTS; ADAPTATION; DROSOPHILA;
D O I
10.3389/fgene.2019.01035
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Recent changes in soybean management like the adoption of transgenic crops and no-till farming, in addition to the expansion of cultivated areas into new virgin frontiers, are some of the hypotheses that can explain the rise of secondary pests, such as the Neotropical brown stink bug, Euschistus heros, in Brazil. To better access the risk of increased pests like E. heros and to determine probabilities for insecticide resistance spreading, it is necessary first to access the levels of the genetic diversity, how the genetic diversity is distributed, and how natural selection is acting upon the natural variation. Using the genotyping by sequencing (GBS) technique, we generated 60,000 single-nucleotide polymorphisms (SNPs) distributed across the E. heros genome to answer some of those questions. The SNP data was used to investigate the pattern of genetic structure, hybridization and natural selection of this emerging pest. We found that E. heros populations presented similar levels of genetic diversity with slightly higher values at several central locations in Brazil. Our results also showed strong genetic structure separating northern and southern Brazilian regions (F-ST = 0.22; p-value = 0.000) with a very distinct hybrid zone at the central region. The analyses also suggest the possibility that GABA channels and odorant receptors might play a role in the process of natural selection. At least one marker was associated with soybean and beans crops, but no association between allele frequency and cotton was found. We discuss the implications of these findings in the management of emerging pests in agriculture, particularly in the context of large areas of monoculture such as soybean and cotton.
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页数:12
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共 68 条
  • [1] Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum (Meyer-Dur)
    An, Xing-Kui
    Sun, Liang
    Liu, Hang-Wei
    Liu, Dan-Feng
    Ding, Yu-Xiao
    Li, Le-Mei
    Zhang, Yong-Jun
    Guo, Yu-Yuan
    [J]. SCIENTIFIC REPORTS, 2016, 6
  • [2] Hybridization and gene flow in the mega-pest lineage of moth, Helicoverpa
    Anderson, Craig J.
    Oakeshott, John G.
    Tay, Wee Tek
    Gordon, Karl H. J.
    Zwick, Andreas
    Walsh, Tom K.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115 (19) : 5034 - 5039
  • [3] LOSITAN:: A workbench to detect molecular adaptation based on a Fst-outlier method
    Antao, Tiago
    Lopes, Ana
    Lopes, Ricardo J.
    Beja-Pereira, Albano
    Luikart, Gordon
    [J]. BMC BIOINFORMATICS, 2008, 9 (1)
  • [4] Rapid SNP Discovery and Genetic Mapping Using Sequenced RAD Markers
    Baird, Nathan A.
    Etter, Paul D.
    Atwood, Tressa S.
    Currey, Mark C.
    Shiver, Anthony L.
    Lewis, Zachary A.
    Selker, Eric U.
    Cresko, William A.
    Johnson, Eric A.
    [J]. PLOS ONE, 2008, 3 (10):
  • [5] THE BARRIER TO GENETIC EXCHANGE BETWEEN HYBRIDIZING POPULATIONS
    BARTON, N
    BENGTSSON, BO
    [J]. HEREDITY, 1986, 57 : 357 - 376
  • [6] ANALYSIS OF HYBRID ZONES
    BARTON, NH
    HEWITT, GM
    [J]. ANNUAL REVIEW OF ECOLOGY AND SYSTEMATICS, 1985, 16 : 113 - 148
  • [7] Evaluating loci for use in the genetic analysis of population structure
    Beaumont, MA
    Nichols, RA
    [J]. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1996, 263 (1377) : 1619 - 1626
  • [8] Comparison of the effects of neonicotinoids and pyrethroids against Oebalus pugnax (Hemiptera: Pentatomidae) in rice
    Blackman, Bryce
    Lanka, Srinivas
    Hummel, Natalie
    Way, Mo
    Stout, Michael
    [J]. FLORIDA ENTOMOLOGIST, 2015, 98 (01) : 18 - 26
  • [9] Assessment of a more conservative stink bug economic threshold for managing stink bugs in Brazilian soybean production
    Bueno, Adeney de Freitas
    Bortolotto, Orcial Ceolin
    Pomari-Fernandes, Aline
    Franca-Net, Jose de Barros
    [J]. CROP PROTECTION, 2015, 71 : 132 - 137
  • [10] Novel GABA receptor pesticide targets
    Casida, John E.
    Durkin, Kathleen A.
    [J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2015, 121 : 22 - 30