Evolution and recombination of genes encoding HIV-1 drug resistance and tropism during antiretroviral therapy

被引：30

作者：

Shi, Binshan ^{[1
]}

Kitchen, Christina ^{[2
]}

Weiser, Barbara ^{[1
,3
]}

Mayers, Douglas ^{[4
]}

Foley, Brian ^{[5
]}

Kemal, Kimdar ^{[1
]}

Anastos, Kathryn ^{[6
]}

Suchard, Marc ^{[2
]}

Parker, Monica ^{[1
]}

Brunner, Cheryl ^{[1
]}

Burger, Harold ^{[1
,3
]}

机构：

[1] New York State Dept Hlth, Wadsworth Ctr, Div Infect Dis, Albany, NY 12208 USA

[2] Univ Calif Los Angeles, Dept Biostat, Los Angeles, CA 90095 USA

[3] Albany Med Coll, Dept Med, Albany, NY 12208 USA

[4] Idenix Pharmaceut Inc, Cambridge, MA 02139 USA

[5] Los Alamos Natl Lab, Los Alamos, NM 87545 USA

[6] Albert Einstein Coll Med, Div Gen Internal Med, Bronx, NY 10467 USA

来源：

VIROLOGY | 2010年 / 404卷 / 01期

关键词：

HIV-1 drug resistance; HIV-1; recombination; tropism; HUMAN-IMMUNODEFICIENCY-VIRUS; FEMALE GENITAL-TRACT; CORECEPTOR USAGE; IN-VIVO; TYPE-1; ENV; V3; LOOP; VIROLOGICAL FAILURE; VIRAL LOAD; POL SEQUENCES; GENOMIC RNA;

D O I：

10.1016/j.virol.2010.04.008

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Characterization of residual plasma virus during antiretroviral therapy (ART) is a high priority to improve understanding of HIV-1 pathogenesis and therapy. To understand the evolution of HIV-1 pot and env genes in viremic patients under selective pressure of ART, we performed longitudinal analyses of plasma-derived pol and env sequences from single HIV-1 genomes. We tested the hypotheses that drug resistance in pol was unrelated to changes in coreceptor usage (tropism), and that recombination played a role in evolution of viral strains. Recombinants were identified by using Bayesian and other computational methods. High-level genotypic resistance was seen in similar to 70% of X4 and R5 strains during ART. There was no significant association between resistance and tropism. Each patient displayed at least one recombinant encompassing env and representing a change in predicted tropism. These data suggest that, in addition to mutation, recombination can play a significant role in shaping HIV-1 evolution. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：5 / 20

页数：16

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