Detection of a mouse OGG1 fragment during caspase-dependent apoptosis: Oxidative DNA damage and apoptosis

被引：19

作者：

Hirano, T

Kawai, K

Ootsuyama, Y

Orimo, H

Kasai, H

机构：

[1] Univ Occupat & Environm Hlth, Sch Med, Dept Environm Oncol, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan

[2] Univ Occupat & Environm Hlth, Sch Med, Dept Dermatol, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan

来源：

CANCER SCIENCE | 2004年 / 95卷 / 08期

关键词：

D O I：

10.1111/j.1349-7006.2004.tb03321.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We investigated the expression of mouse 8-oxoguanine DNA glycosylase 1 (mOGG1) in mouse non-parenchymal hepatocytes (NCTC) during etoposide- or mitomycin C (MMC)-induced apoptosis. We observed mOGG1 fragmentation in apoptotic cells. The apoptosis accompanying the fragmentation of mOGG1 was caspase-dependent. The mOGG1 fragment existed in both the cytoplasm and nucleus of the etoposide-treated NCTC, indicating that the mOGG1 fragment could be transferred into the nucleus. In addition, 8-hydroxyguanine (8-OH-Gua, 7,8-dihydro-8-oxoguanine) accumulated in the DNA of NCTC treated with etoposide, suggesting that the mOGG1 fragment might not function as a normal repair enzyme in etoposide-treated NCTC. Although we have not clarified in detail the mechanism and the significance of the mOGG1 fragmentation, further study of the fragmentation of DNA repair enzymes might provide insights into the relationship between oxidative DNA damage and apoptosis.

引用

页码：634 / 638

页数：5

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