Production of hairy roots in Aconitum heterophyllum wall. using Agrobacterium rhizogenes

被引：0

作者：

Giri, A

Banerjee, S

Ahuja, PS

Giri, CC ^{[1
]}

机构：

[1] CIMAP, Plant Tissue Culture Div, Lucknow 226015, Uttar Pradesh, India

[2] Osmania Univ, Dept Genet, Ctr Plant Mol Biol, Hyderabad 500007, Andhra Pradesh, India

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 1997年 / 33卷 / 04期

关键词：

Aconitum heterophyllum; Agrobacterium rhizogenes; alkaloid content; transformed roots;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A method for the production of hairy roots of Aconitum heterophyllum wall. is reported for the first time. Embryogenic callus cultures were successfully transformed using Agrobacterium rhizogenes strains viz. LBA 9402, LBA 9360, and A4 for the induction of hairy roots. The transgenic nature of hairy roots was confirmed by mannopine assay using paper electrophoresis. Best growth of transformed roots was obtained on 1/4 MS (Murashige and Skoog, 1962) medium with 3% sucrose. Total alkaloid (aconites) content of transformed roots was 2.96%, which was 3.75 times higher compared to 0.79% in the nontransformed (control) roots. Thin layer chromatography (TLC) analysis of the components of aconites in the transformed roots revealed the presence of heteratisine, atisine, and hetidine.

引用

页码：280 / 284

页数：5

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