Receptor subtype involved in α1-adrenergic receptor-mediated Ca2+ signaling in cardiomyocytes

Aim: The enhancement of intracellular Ca2+ signaling in response to alpha(1)-adrenergic receptor (alpha(1)-AR) stimulation is an essential signal transduction event in the regulation of cardiac functions, such as cardiac growth, cardiac contraction, and cardiac adaptation to various situations. The present study was intended to determine the role(s) of the alpha(1)-AR subtype(s) in mediating this response. Methods: We evaluated the effects of subtype-specific agonists and antagonists of the alpha(1)-AR on the intracellular Ca2+ signaling of neonatal rat ventricular myocytes using a confocal microscope. Results: After being cultured for 48 h, the myocytes exhibited spontaneous local Ca2+ release, sparks, and global Ca2+ transients. The activation of the alpha(1)-AR with phenylephrine, a selective agonist of the alpha(1)-AR, dose-dependently increased the frequency of Ca2+ transients with an EC50 value of 2.3 mu mol/L. Blocking the alpha(1A)-AR subtype with 5-methylurapidil (5-Mu) inhibited the stimulatory effect of phenylephrine with an IC50 value of 6.7 nmol/L. In contrast, blockade of the alpha(1B)-AR and alpha(1D)-AR subtypes with chlorethylclonidine and BMY 7378, respectively, did not affect the phenylephrine effect. Similarly, the local Ca2+ spark numbers were also increased by the activation of the alpha(1)-AR, and this effect could be abolished selectively by 5-Mu. More importantly, A61603, a novel selective alpha(1A)-AR agonist, mimicked the effect of phenylephrine, but with more potency (EC50 value=6.9 nmol/L) in the potentiation of Ca2+ transients, and blockade of the alpha(1A)-AR by 5-Mu caused abolishment of its effects. Conclusion: These results indicate that alpha(1)-adrenergic stimulation of intracellular Ca2+ activity is mediated selectively by the alpha(1A)-AR.