Riboflavin-ultraviolet light pathogen reduction treatment does not impact the immunogenicity of murine red blood cells

被引：7

作者：

Tormey, Christopher A. ^{[1
,3
]}

Santhanakrishnan, Manjula ^{[1
]}

Smith, Nicole H. ^{[4
]}

Liu, Jingchun ^{[1
]}

Marschner, Susanne ^{[5
]}

Goodrich, Raymond P. ^{[5
]}

Hendrickson, Jeanne E. ^{[1
,2
,4
]}

机构：

[1] Yale Univ, Sch Med, Dept Lab Med, New Haven, CT 06510 USA

[2] Yale Univ, Sch Med, Dept Pediat, New Haven, CT 06510 USA

[3] VA Connecticut Healthcare Syst, West Haven, CT USA

[4] Emory Univ, Sch Med, Dept Pediat & Pathol, Atlanta, GA USA

[5] Terumo BCT, Lakewood, CO USA

来源：

TRANSFUSION | 2016年 / 56卷 / 04期

关键词：

GREEN FLUORESCENT PROTEIN; PREVENTS ALLOIMMUNIZATION; IMMUNE-RESPONSE; CARDIAC-SURGERY; INACTIVATION; TRANSFUSION; PLATELETS; ANTIGEN; PLASMA; SAFETY;

D O I：

10.1111/trf.13432

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

BACKGROUNDUltraviolet (UV) illumination/pathogen reduction effectively inactivates white blood cells (WBCs) in whole blood. Given that cotransfused WBCs may impact recipient immune responses, we hypothesized that pathogen reduction of whole blood may alter responses to RBC antigens. STUDY DESIGN AND METHODSTransgenic mice expressing a model (HOD) antigen, authentic human (hGPA or KEL) antigens, or natural fluorescence (uGFP) on their RBCs were utilized as blood donors. Recipients were transfused with fresh whole blood to which riboflavin had been added or fresh whole blood treated by UV illumination/pathogen reduction treatment after the addition of riboflavin. Posttransfusion RBC recovery, survival, and alloimmunization were measured by flow cytometry. RESULTSUV illumination/pathogen reduction treatment did not alter RBC antigen expression, and recipients of treated syngeneic RBCs had persistently negative direct antiglobulin tests. Greater than 75% of treated and untreated syngeneic RBCs were recovered 24 hours posttransfusion in all experiments, although alterations in the long-term posttransfusion survival of treated RBCs were observed. Treated and untreated KEL RBCs induced similar recipient alloimmune responses, with all recipients making anti-KEL glycoprotein immunoglobulins (p>0.05). Alloimmune responses to treated HOD or hGPA RBCs were no different from untreated RBCs (p>0.05). CONCLUSIONPathogen inactivation treatment of fresh whole murine blood with riboflavin and UV illumination does not impact the rate or magnitude of RBC alloimmunization to three distinct RBC antigens. Further, UV illumination/pathogen reduction appears safe from an immunohematologic standpoint, with no immunogenic neoantigens detected on treated murine RBCs. Future studies with fresh and stored human RBCs are warranted to confirm these findings.

引用

页码：863 / 872

页数：10

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