Microchip-based capillary electrophoresis for determination of lactate dehydrogenase isoenzymes

被引:8
作者
Zhuang, Gui-Sheng
Liu, Jing
Jia, Chun-Ping
Jin, Qing-Hui
Zhao, Jian-Long
Wang, Hui-Min
机构
[1] Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, State Key Lab Transducer Technol, Shanghai 200050, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
[3] Nantong Univ, Affiliated Hosp, Nantong, Jiangsu, Peoples R China
关键词
capillary electrophoresis; isoenzymes; lactate dehydrogenase; microchip;
D O I
10.1002/jssc.200600506
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This article describes a novel microchip-based capillary electrophoresis and on-column enzymatic reaction analysis protocol for lactate dehydrogenase (LDH) isoenzymes with a home-made xenon lamp-induced fluorescence detection system. A microchip integrated with a temperature-control unit is designed and fabricated for low-temperature electrophoretic separation of LDH isoenzymes, optimal enzyme reaction temperature control, and product detection. A four-step operation and temperature control are employed for the determination of LDH activity by on-chip monitoring of the amount of incubation product of NADH during the fixed incubation period and at a fixed temperature. Experiments on the determination of LDH standard sample and serum LDH isoenzymes from a healthy adult donor are carried out. The results are comparable with those obtained by conventional CE. Shorter analysis times and a more stable and lower background baseline can be achieved. The efficient separation of different LDH forms indicates the potential of microfluidic devices for isoenzyme assay.
引用
收藏
页码:1350 / 1356
页数:7
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