Apoptosis inducing effects of 6-methoxydihydrosanguinarine in HT29 colon carcinoma cells

被引：21

作者：

Lee, YJ

Yin, HQ

Kim, YH

Li, GY

Lee, BH

机构：

[1] Wonkwang Univ, Coll Pharm, Iksan 570749, Jeonbuk, South Korea

[2] Wonkwang Univ, MeRRI, Iksan 570749, Jeonbuk, South Korea

[3] Chungnam Natl Univ, Coll Pharm, Taejon, South Korea

来源：

ARCHIVES OF PHARMACAL RESEARCH | 2004年 / 27卷 / 12期

关键词：

6-methoxydihydrosanguinarine; Hylomecon hylomeconoides; apoptosis; HT29;

D O I：

10.1007/BF02975890

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

6-Methoxydihydrosanguinarine (6WE), a benzophenanthridine alkaloid derived from the methanol extracts of Hylomecon hylomeconoides, showed a dose-dependent effect at 1-10 muM on causing apoptotic cell death in HT29 colon carcinoma cells (IC50 = 5.0 +/- 0.2 muM). Treatment of HT-29 cells with 6ME resulted in the formation of internucleosomal DNA fragmentation. Treatment of the cells with 6ME caused activation of caspase-3, -8 and 9 protease and subsequent proteolytic cleavage of poly(ADP-ribose)polymerase. 6ME increased the expression of p53 and Bax and decreased the expression of Bid. These results indicate that p53 and proapoptotic Bcl-2 family proteins might participate in the antiproliferative activity of 6ME in HT29 cells.

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页码：1253 / 1257

页数：5

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