Transformation of chickpea: effect of genotype, explant, Agrobacterium-strain and composition of culture medium

被引：15
|
作者：
Bhattacharjee, B. [1 ]
Mohan, M. [1 ]
Nair, S. [1 ]
机构：
[1] Int Ctr Genet Engn & Biotechnol, New Delhi 110067, India
来源：
BIOLOGIA PLANTARUM | 2010年 / 54卷 / 01期
关键词：
Cicer arietinum; cotyledon- and cotyledonary node-derived calli; beta-glucuronidase; hygromycin phosphotransferase; CICER-ARIETINUM L; ARACHIS-HYPOGAEA L; MEDIATED TRANSFORMATION; GENETIC-TRANSFORMATION; PLANT-REGENERATION; SHOOT REGENERATION; EFFICIENT PROTOCOL; TUMEFACIENS; DNA; EXPRESSION;
D O I：
10.1007/s10535-010-0004-4
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Reproducible and high-frequency transgenic plant regeneration from callus and embryo axes of four different genotypes of chickpea (Cicer arietinum) was achieved after Agrobacterium-mediated transformation. Three different strains of Agrobacterium (EHA105, AGL1 and LBA4404) harboring the binary vector pCAMBIA1301 containing beta-glucuronidase (GUS) and hygromycin phosphotransferase (hpt) genes under the control of a CaMV35S promoter were used. The highest number of transgenic plants was obtained from cotyledonary node-derived calli of genotype Pusa-256. A highly efficient rooting was achieved on Murashige and Skoog medium supplemented with indole-3-butyric acid. The stable integration of the gene was confirmed by molecular analyses of the transformed plants. Inheritance of GUS and hpt gene was followed through two generations and they showed the expected 3:1 inheritance.
引用
收藏
页码：21 / 32
页数：12
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