Study of the cashew gum polysaccharide for the horseradish peroxidase immobilization - Structural characteristics, stability and recovery

被引：39

作者：

Silva, Thamara M. ^{[1
]}

Santiago, Patricia O. ^{[1
]}

Purcena, Luiza L. A. ^{[1
]}

Fernandes, Katia F. ^{[1
]}

机构：

[1] Univ Fed Goias, Inst Ciencias Biol, Lab Quim Proteinas, BR-74001970 Goiania, Go, Brazil

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2010年 / 30卷 / 04期

关键词：

Cashew gum; Immobilization; Horseradish peroxidase; Stability; Organic solvents; ENZYME ELECTRODE;

D O I：

10.1016/j.msec.2010.01.016

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

This work describes the use of the cashew gum polysaccharide (CGP) for the immobilization of the horseradish peroxidase and its recovery from the reaction medium by precipitation with organic solvents. The partial characterization of the CGP (FT-IR, DSC, and protein content) and the importance of the proteins for the stabilization of CGP are also presented. The covalent immobilization of the HRP was optimized and it reached a yield of 25.4% when 98.2 U of HRP was added to 15 mg of CGP activated with glutaraldehyde. Best results of reuse were obtained using acetone to precipitate the complex CGPg-HRP, with more than 95% of activity in the 5 cycle of use. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：526 / 530

页数：5

共 38 条

[1] Immobilization of tyrosinase in chitosan film for an optical detection of phenol [J].

Abdullah, J ;

Ahmad, M ;

Karuppiah, N ;

Heng, LY ;

Sidek, H .

SENSORS AND ACTUATORS B-CHEMICAL, 2006, 114 (02) :604-609

[2] STUDIES OF URONIC ACID MATERIALS .48. STRUCTURAL-ANALYSIS OF GUM POLYSACCHARIDE FROM ANACARDIUM-OCCIDENTALE [J].

ANDERSON, DMW ;

BELL, PC .

ANALYTICA CHIMICA ACTA, 1975, 79 (OCT) :185-197

[3] Detection of three wound-induced proteins in papaya latex [J].

Azarkan, M ;

Wintjens, R ;

Looze, Y ;

Baeyens-Volant, D .

PHYTOCHEMISTRY, 2004, 65 (05) :525-534

[4] Infrared spectroscopy of proteins [J].

Barth, Andreas .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2007, 1767 (09) :1073-1101

[5] Hybrid organic-inorganic sensors [J].

Bescher, E ;

Mackenzie, JD .

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 1998, 6 (2-3) :145-154

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7]

CABRAL JMS, 2003, LIDEL EDICOES TECNIC, P141

[8] Enzymatic immobilisation in kappa-carrageenan gel suitable for organic phase enzyme electrode (OPEE) assembly [J].

Campanella, L ;

Favero, G ;

Sammartino, MP ;

Tomassetti, M .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 1999, 7 (1-4) :101-113

[9] Covalent immobilisation of horseradish peroxidase onto poly (ethylene terephthalate)-poly (aniline) composite [J].

Caramori, SS ;

Fernandes, KF .

PROCESS BIOCHEMISTRY, 2004, 39 (07) :883-888

[10] Synthesis and characterization of cashew gum/acrylic acid nanoparticles [J].

da Silva, Durcilene A. ;

Feitosa, Judith P. A. ;

Paula, Haroldo C. B. ;

de Paula, Regina C. M. .

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 2009, 29 (02) :437-441

← 1 2 3 4 →