Protection of Fibroblasts (NIH-3T3) against Oxidative Damage by Cyanidin-3-rhamnoglucoside Isolated from Fig Fruits (Ficus carica L.)

被引：25

作者：

Solomon, Anat ^{[2
,4
]}

Golubowicz, Sara ^{[2
]}

Yablowicz, Zeev ^{[2
]}

Bergman, Margalit ^{[3
]}

Grossman, Shlomo ^{[3
]}

Altman, Arie ^{[4
]}

Kerem, Zohar ^{[1
]}

Flaishman, Moshe A. ^{[2
]}

机构：

[1] Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, Inst Biochem Food Sci & Nutr, IL-76100 Rehovot, Israel

[2] Agr Res Org, Volcani Ctr, Inst Plant Sci, Dept Fruit Tree Sci, IL-50250 Bet Dagan, Israel

[3] Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel

[4] Hebrew Univ Jerusalem, Robert H Smith Inst Plant Sci & Genet Agr, IL-76100 Rehovot, Israel

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2010年 / 58卷 / 11期

关键词：

Fig fruits; cyanidin-3-rhamnoglucoside (C3R); ROS; NIH-3T3; fibroblasts; Fe2+ chelation; linoleic acid oxidation; lysosome membrane stability; ANTIOXIDANT ACTIVITY; MEMBRANE DAMAGE; ANTHOCYANINS; INHIBITION; FLAVONOIDS; PRODUCTS; CYANIDIN; FRESH; MALONALDEHYDE; PEROXIDATION;

D O I：

10.1021/jf100122a

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Anthocyanins, plant secondary metabolites, have been recognized for their health-promoting properties when consumed by humans. In this study, the antioxidant properties of a major anthocyanin in fresh fig fruits, cyanidin-3-rhamnoglucoside (C3R), were evaluated by various assays in vitro and correlated with the protection afforded by C3R to cultured NIH-3T3 fibroblast cells. C3R inhibited lipid peroxidation from producing peroxy radicals (ROO center dot) and MDA in a dose-dependent manner, and a high calculated stoichiometric coefficient [n] for peroxy radicals was demonstrated. In addition to its scavenging of reactive oxygen species (ROS), C3R showed a strong chelating activity toward the Fe2+ ion. Finally, pretreatment with C3R inhibited proapoptotic processes that were initiated by the oxidation of lysosome membranes in fibroblast cells. The high antioxidant potential, with several modes of action of purified C3R, may contribute to health benefits gained by the consumption of fresh fig fruits.

引用

页码：6660 / 6665

页数：6

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