A Spectrophotometric Assay for Robust Viability Testing of Seed Batches Using 2,3,5-Triphenyl Tetrazolium Chloride: Using Hordeum vulgare L. as a Model

被引:36
作者
Del Egido, Laura Lopez [1 ,2 ,4 ]
Navarro-Miro, David [1 ,5 ]
Martinez-Heredia, Victor [1 ,6 ]
Toorop, Peter E. [3 ]
Iannetta, Pietro P. M. [1 ]
机构
[1] James Hutton Inst, Ecol Sci, Dundee, Scotland
[2] Univ Pavia, Dept Earth & Environm Sci, Pavia, Italy
[3] Royal Bot Gardens, Comparat Plant & Fungal Biol Dept, Richmond, England
[4] Syngenta Seeds BV, Seed Technol, Enkhuizen, Netherlands
[5] Univ Barcelona, Fac Biol, Inst Recerca Biodiversitat, Dept Biol Evolut Ecol & Ciencies Ambientals,Grp R, Barcelona, Spain
[6] Dundee Univ, James Hutton Inst, Div Plant Sci, Dundee, Scotland
来源
FRONTIERS IN PLANT SCIENCE | 2017年 / 8卷
基金
欧盟第七框架计划;
关键词
formazan; Hordeum vulgare; seed-testing; seed viability; tetrazolium; TTC; GERMINATION; TEMPERATURE; REDUCTION; STORAGE; SYSTEM; SPP;
D O I
10.3389/fpls.2017.00747
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A comparative analysis was carried out of published methods to assess seed viability using 2,3,5-triphenyltetrazolium chloride (TTC) based assays of seed batches. The tests were carried out on seeds of barley (Hordeum vulgare cv. Optic) as a model. We established that 10% [w/v] trichloroacetic acid (TCA)/methanol is superior to the acetone and methanol-only based methods: allowing the highest recovery of formazan and the lowest background optical density (OD) readings, across seed lots comprising different ratios of viable and dead seeds. Themethod allowed a linear-model to accurately capture the statistically significant relationship between the quantity of formazan that could be extracted using the method we developed and the seed temperature-response, and seed viability as a function of artificially aged seed lots. Other quality control steps are defined to help ensure the assay is robust and these are reported in a Standard Operating Procedure.
引用
收藏
页数:9
相关论文
共 36 条
  • [1] Booer C., 2001, MALTING BREWING CHAR
  • [2] Brar J, 2013, INDIAN J EXP BIOL, V51, P88
  • [3] Assessing root death and root system dynamics in a study of grape canopy pruning
    Comas, LH
    Eissenstat, DM
    Lakso, AN
    [J]. NEW PHYTOLOGIST, 2000, 147 (01) : 171 - 178
  • [4] Copeland L. O., 2001, PRINCIPLES SEED SCI, DOI [10.1007/978-1-4615-1619-4_6, DOI 10.1007/978-1-4615-1619-4_]
  • [5] Improved tetrazolium viability testing in orchid seeds with a thick carapace (Dactylorhiza fuchsii) or dark seed coat (Vanda curvifolia)
    Custodio, C. C.
    Marks, T. R.
    Pritchard, H. W.
    Hosomi, S. T.
    Machado-Neto, N. B.
    [J]. SEED SCIENCE AND TECHNOLOGY, 2016, 44 (01) : 177 - 188
  • [6] Tetrazolium test for the viability of gherkin seeds
    de Paiva, Emanoela Pereira
    Torres, Salvador Barros
    Nobre de Almeida, Joao Paulo
    da Silva Sa, Francisco Vanies
    Torquato Oliveira, Renata Ramayane
    [J]. REVISTA CIENCIA AGRONOMICA, 2017, 48 (01): : 118 - 124
  • [7] Parreño-de Guzman LE, 2011, PHILIPP J CROP SCI, V36, P10
  • [8] Preconditioning of castor bean seeds for the tetrazolium test
    Gaspar-Oliveira, Carolina Maria
    Martins, Cibele Chalita
    Nakagawa, Joao
    [J]. ACTA SCIENTIARUM-AGRONOMY, 2011, 33 (02) : 303 - 311
  • [9] Effects of tetrazolium chloride concentration, O2′ and cell age on dehydrogenase activity of Aspergillus niger
    Ghaly, A. E.
    Mahmoud, N. S.
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2007, 136 (02) : 207 - 222
  • [10] Grzybowski Camila Ribeiro de Souza, 2012, Rev. bras. sementes, V34, P47, DOI 10.1590/S0101-31222012000100006