Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems

被引：37
作者：
Garcia-Alonso, Javier [1 ]
Fakhrullin, Rawil F. [2 ,3 ]
Paunov, Vesselin N. [3 ]
机构：
[1] Univ Hull, Dept Biol Sci, Kingston Upon Hull HU6 7RX, N Humberside, England
[2] Kazan VI Lenin State Univ, Dept Biochem, Kazan 420008, Russia
[3] Univ Hull, Dept Chem, Kingston Upon Hull HU6 7RX, N Humberside, England
来源：
BIOSENSORS & BIOELECTRONICS | 2010年 / 25卷 / 07期
关键词：
Cell magnetization; GFP; Toxicity screening; Magnetic nanoparticles; SACCHAROMYCES-CEREVISIAE; GENOTOXICITY ASSAY; CELLS; NANOPARTICLES; CULTURE; REMOVAL;
D O I：
10.1016/j.bios.2009.11.016
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Saccharomyces cerevisiae containing fluorescent markers are ideal candidates for applications in microfluidic screening systems as fluorescence signal is emitted without the need of additional reagents. Here we develop a method for magnetic functionalisation of such cells which allows their handling and immobilization in micro-screening devices. After exposure of the magnetized GFP-reporter yeast (GreenScreen(TM)) to a genotoxic compound, the fluorescence emission was detected using fluorescent spectrometer and an Epi-fluorescent microscope. Results demonstrate that GFP production and fluorescence emission is not altered by their magnetic functionalization. indicating its potential employment on biosensors, bioreactors and micro-screening studies. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：1816 / 1819
页数：4
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