Foxc2 and BMP2 Induce Osteogenic/Odontogenic Differentiation and Mineralization of Human Stem Cells from Apical Papilla

被引：18

作者：

Zhang, Wen ^{[1
,2
]}

Zhang, Xiaolei ^{[1
,2
]}

Li, Junyuan ^{[3
]}

Zheng, Jianmao ^{[1
,2
]}

Hu, Xiaoli ^{[1
,2
]}

Xu, Meng ^{[1
,2
]}

Mao, Xueli ^{[1
,2
]}

Ling, Junqi ^{[1
,2
]}

机构：

[1] Sun Yat Sen Univ, Guanghua Sch, Guangdong Prov Key Lab Stomatol, Guangzhou 510055, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Hosp Stomatol, Guangzhou 510055, Guangdong, Peoples R China

[3] Jinan Univ, Affiliated Hosp 1, Med Ctr Stomatol, Guangzhou 510630, Guangdong, Peoples R China

来源：

STEM CELLS INTERNATIONAL | 2018年 / 2018卷

基金：

中国国家自然科学基金;

关键词：

TRANSCRIPTION FACTOR; ODONTOGENIC DIFFERENTIATION; NEURAL CREST; PROLIFERATION; REGENERATION; EXPRESSION; PATHWAY;

D O I：

10.1155/2018/2363917

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

As a transcription factor regulated by bone morphogenetic protein 2 (BMP2), Forkhead c2 (Foxc2) plays a pivot role in osteogenesis/odontogenesis. However, the role of Foxc2 and BMP2 in regulating osteo-/odontogenic differentiation and mineralization of stem cells from apical papilla (SCAP) is still uncertain. In this research, overexpression of Foxc2 gene significantly improved the proliferation of SCAP four days and eight days after transfection, but overexpression of both Foxc2 and BMP2 genes significantly inhibited the proliferation of SCAP eight days after transfection. RT-qPCR and western blot results indicated that SCAP-Foxc2-BMP2 significantly upregulated osteo-/odontogenic genes and proteins at most of the time points in SCAP after transfection. Moreover, SCAP-Foxc2-BMP2 formed notably more alkaline phosphatase-positive and alizarin red-positive mineralized nodules than other three group cells sixteen days after transfection. In conclusion, our findings revealed that Foxc2 and BMP2 synergistically promoted osteo-/odontogenic differentiation and mineralization of SCAP in vitro.

引用

页数：10

共 34 条

[21] Foxc1 and Foxc2 in the Neural Crest Are Required for Ocular Anterior Segment Development [J].