Foxc2 and BMP2 Induce Osteogenic/Odontogenic Differentiation and Mineralization of Human Stem Cells from Apical Papilla

被引:18
作者
Zhang, Wen [1 ,2 ]
Zhang, Xiaolei [1 ,2 ]
Li, Junyuan [3 ]
Zheng, Jianmao [1 ,2 ]
Hu, Xiaoli [1 ,2 ]
Xu, Meng [1 ,2 ]
Mao, Xueli [1 ,2 ]
Ling, Junqi [1 ,2 ]
机构
[1] Sun Yat Sen Univ, Guanghua Sch, Guangdong Prov Key Lab Stomatol, Guangzhou 510055, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Hosp Stomatol, Guangzhou 510055, Guangdong, Peoples R China
[3] Jinan Univ, Affiliated Hosp 1, Med Ctr Stomatol, Guangzhou 510630, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
TRANSCRIPTION FACTOR; ODONTOGENIC DIFFERENTIATION; NEURAL CREST; PROLIFERATION; REGENERATION; EXPRESSION; PATHWAY;
D O I
10.1155/2018/2363917
中图分类号
Q813 [细胞工程];
学科分类号
摘要
As a transcription factor regulated by bone morphogenetic protein 2 (BMP2), Forkhead c2 (Foxc2) plays a pivot role in osteogenesis/odontogenesis. However, the role of Foxc2 and BMP2 in regulating osteo-/odontogenic differentiation and mineralization of stem cells from apical papilla (SCAP) is still uncertain. In this research, overexpression of Foxc2 gene significantly improved the proliferation of SCAP four days and eight days after transfection, but overexpression of both Foxc2 and BMP2 genes significantly inhibited the proliferation of SCAP eight days after transfection. RT-qPCR and western blot results indicated that SCAP-Foxc2-BMP2 significantly upregulated osteo-/odontogenic genes and proteins at most of the time points in SCAP after transfection. Moreover, SCAP-Foxc2-BMP2 formed notably more alkaline phosphatase-positive and alizarin red-positive mineralized nodules than other three group cells sixteen days after transfection. In conclusion, our findings revealed that Foxc2 and BMP2 synergistically promoted osteo-/odontogenic differentiation and mineralization of SCAP in vitro.
引用
收藏
页数:10
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共 34 条
[1]   Foxc2CreERT2 knock-in mice mark stage-specific Foxc2-expressing cells during mouse organogenesis [J].
Amin, Mohammed Badrul ;
Miura, Naoyuki ;
Uddin, Mohammad Khaja Mafij ;
Islam, Mohammod Johirul ;
Yoshida, Nobuaki ;
Iseki, Sachiko ;
Kume, Tsutomu ;
Trainor, Paul A. ;
Saitsu, Hirotomo ;
Aoto, Kazushi .
CONGENITAL ANOMALIES, 2017, 57 (01) :24-31
[2]   Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application [J].
Bakopoulou, Athina ;
About, Imad .
STEM CELLS INTERNATIONAL, 2016, 2016
[3]   Tissue Interactions Regulating Tooth Development and Renewal [J].
Balic, Anamaria ;
Thesleff, Irma .
CRANIOFACIAL DEVELOPMENT, 2015, 115 :157-186
[4]   Foxc2 enhances proliferation and inhibits apoptosis through activating Akt/mTORC1 signaling pathway in mouse preadipocytes [J].
Gan, Lu ;
Liu, Zhenjiang ;
Jin, Wei ;
Zhou, Zhongjie ;
Sun, Chao .
JOURNAL OF LIPID RESEARCH, 2015, 56 (08) :1471-1480
[5]   Current Advance and Future Prospects of Tissue Engineering Approach to Dentin/Pulp Regenerative Therapy [J].
Gong, Ting ;
Heng, Boon Chin ;
Lo, Edward Chin Man ;
Zhang, Chengfei .
STEM CELLS INTERNATIONAL, 2016, 2016
[6]   Comparison of the differentiation potential of neural crest derived progenitor cells from apical papilla (dNC-PCs) and stem cells from exfoliated deciduous teeth (SHED) into mineralising cells [J].
Gosau, Martin ;
Goetz, Werner ;
Felthaus, Oliver ;
Ettl, Tobias ;
Jaeger, Andreas ;
Morsczeck, Christian .
ARCHIVES OF ORAL BIOLOGY, 2013, 58 (06) :699-706
[7]   Foxc2 induces Wnt4 and Bmp4 expression during muscle regeneration and osteogenesis [J].
Gozo, M. C. ;
Aspuria, P-J ;
Cheon, D-J ;
Walts, A. E. ;
Berel, D. ;
Miura, N. ;
Karlan, B. Y. ;
Orsulic, S. .
CELL DEATH AND DIFFERENTIATION, 2013, 20 (08) :1031-1042
[8]   Mesenchymal Stem Cells Derived from Dental Tissues vs. Those from Other Sources: Their Biology and Role in Regenerative Medicine [J].
Huang, G. T. -J. ;
Gronthos, S. ;
Shi, S. .
JOURNAL OF DENTAL RESEARCH, 2009, 88 (09) :792-806
[9]   The hidden treasure in apical papilla: The potential role in pulp/dentin regeneration and bioroot engineering [J].
Huang, George T. -J. ;
Sonoyama, Wataru ;
Liu, Yi ;
Liu, He ;
Wang, Songlin ;
Shi, Songtao .
JOURNAL OF ENDODONTICS, 2008, 34 (06) :645-651
[10]   Signaling Networks Regulating Tooth Organogenesis and Regeneration, and the Specification of Dental Mesenchymal and Epithelial Cell Lineages [J].
Jussila, Maria ;
Thesleff, Irma .
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2012, 4 (04)