In vivo bioluminescence and reflectance imaging of multiple organs in bioluminescence reporter mice by bundled-fiber-coupled microscopy

被引：8

作者：

Ando, Yoriko ^{[1
]}

Sakurai, Takashi ^{[1
,2
]}

Koida, Kowa ^{[1
,3
]}

Tei, Hajime ^{[4
]}

Hida, Akiko ^{[5
]}

Nakao, Kazuki ^{[6
,7
]}

Natsume, Mistuo ^{[8
]}

Numano, Rika ^{[1
,9
]}

机构：

[1] Toyohashi Univ Technol, Elect Inspired Interdisciplinary Res Inst EIIRIS, Toyohashi, Aichi 4418580, Japan

[2] Juntendo Univ, Bunkyo Ku, Tokyo 1138421, Japan

[3] Toyohashi Univ Technol, Dept Comp Sci & Engn, Toyohashi, Aichi 4418580, Japan

[4] Kanazawa Univ, Grad Sch Nat Sci & Technol, Kanazawa, Ishikawa 9201192, Japan

[5] Natl Inst Mental Hlth, Dept Psychophysiol, Natl Ctr Neurol & Psychiat, Kodaira, Tokyo 1878553, Japan

[6] Univ Tokyo, Ctr Dis Biol & Integrat Med, Lab Anim Resources,Grad Sch Med, Bunkyo Ku, Tokyo 1130033, Japan

[7] RIKEN, Ctr Dev Biol, Lab Anim Resources & Genet Engn, Kobe, Hyogo 6500047, Japan

[8] Denkosha Co Ltd, Hamamatsu, Shizuoka 4328055, Japan

[9] Toyohashi Univ Technol, Dept Environm & Life Sci, Biol Regulatory Engn, Toyohashi, Aichi 4418580, Japan

来源：

BIOMEDICAL OPTICS EXPRESS | 2016年 / 7卷 / 03期

关键词：

DROSOPHILA PERIOD GENE; FLUORESCENCE MICROENDOSCOPY; CIRCADIAN OSCILLATORS; LUMINESCENT PROTEINS; NEURAL ACTIVITY; CLOCK GENES; D-LUCIFERIN; EXPRESSION; SYSTEM; MOUSE;

D O I：

10.1364/BOE.7.000963

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bioluminescence imaging (BLI) is used in biomedical research to monitor biological processes within living organisms. Recently, fiber bundles with high transmittance and density have been developed to detect low light with high resolution. Therefore, we have developed a bundled-fiber-coupled microscope with a highly sensitive cooled-CCD camera that enables the BLI of organs within the mouse body. This is the first report of in vivo BLI of the brain and multiple organs in luciferase-reporter mice using bundled-fiber optics. With reflectance imaging, the structures of blood vessels and organs can be seen clearly with light illumination, and it allowed identification of the structural details of bioluminescence images. This technique can also be applied to clinical diagnostics in a low invasive manner. (C) 2016 Optical Society of America

引用

页码：963 / 978

页数：16

共 47 条

[11] Fiber bundle endocytoscopy [J].