Application of CRISPR-Cas9 Based Genome-Wide Screening Approaches to Study Cellular Signalling Mechanisms

被引：38

作者：

Sharma, Sumana ^{[1
,2
]}

Petsalaki, Evangelia ^{[1
]}

机构：

[1] European Bioinformat Inst, European Mol Biol Lab, Wellcome Genome Campus, Cambridge CB10 1SD, England

[2] Wellcome Sanger Inst, Cell Surface Signalling Lab, Wellcome Genome Campus, Cambridge CB10 1SD, England

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2018年 / 19卷 / 04期

关键词：

CRISPR/Cas9; genome-wide screens; cellular signalling; ESSENTIAL GENES; TRANSCRIPTIONAL ACTIVATION; REGULATORY CIRCUITS; KNOCKOUT; IDENTIFICATION; NETWORKS; VIRUS; VULNERABILITIES; SUPPRESSORS; SENSITIVITY;

D O I：

10.3390/ijms19040933

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cellular signalling process is a highly complex mechanism, involving multiple players, which together orchestrate the cell's response to environmental changes and perturbations. Given the multitude of genes that participate in the process of cellular signalling, its study in a genome-wide manner has proven challenging. Recent advances in gene editing technologies, including clustered regularly-interspaced short palindromic repeats/Cas9 (CRISPR/Cas9) approaches, have opened new opportunities to investigate global regulatory signalling programs of cells in an unbiased manner. In this review, we focus on how the application of pooled genetic screening approaches using the CRISPR/Cas9 system has contributed to a systematic understanding of cellular signalling processes in normal and disease contexts.

引用

页数：14

共 78 条

[1] A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response [J].

Adamson, Britt ;

Norman, Thomas M. ;

Jost, Marco ;

Cho, Min Y. ;

Nunez, James K. ;

Chen, Yuwen ;

Villalta, Jacqueline E. ;

Gilbert, Luke A. ;

Horlbeck, Max A. ;

Hein, Marco Y. ;

Pak, Ryan A. ;

Gray, Andrew N. ;

Gross, Carol A. ;

Dixit, Atray ;

Parnas, Oren ;

Regev, Aviv ;

Weissman, Jonathan S. .

CELL, 2016, 167 (07) :1867-+

[2] Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting [J].

Aguirre, Andrew J. ;

Meyers, Robin M. ;

Weir, Barbara A. ;

Vazquez, Francisca ;

Zhang, Cheng-Zhong ;

Ben-David, Uri ;

Cook, April ;

Ha, Gavin ;

Harrington, William F. ;

Doshi, Mihir B. ;

Kost-Alimova, Maria ;

Gill, Stanley ;

Xu, Han ;

Ali, Levi D. ;

Jiang, Guozhi ;

Pantel, Sasha ;

Lee, Yenarae ;

Goodale, Amy ;

Cherniack, Andrew D. ;

Oh, Coyin ;

Kryukov, Gregory ;

Cowley, Glenn S. ;

Garraway, Levi A. ;

Stegmaier, Kimberly ;

Roberts, Charles W. ;

Golub, Todd R. ;

Meyerson, Matthew ;

Root, David E. ;

Tsherniak, Aviad ;

Hahn, William C. .

CANCER DISCOVERY, 2016, 6 (08) :914-929

[3] Strategies to discover regulatory circuits of the mammalian immune system [J].

Amit, Ido ;

Regev, Aviv ;

Hacohen, Nir .

NATURE REVIEWS IMMUNOLOGY, 2011, 11 (12) :873-880

[4]

Berridge MJ, 2014, CELL SIGNAL BIOL, V6, pcsb0001002, DOI 10.1042/csb0001002

[5] Integrating physical and genetic maps: from genomes to interaction networks [J].

Beyer, Andreas ;

Bandyopadhyay, Sourav ;

Ideker, Trey .

NATURE REVIEWS GENETICS, 2007, 8 (09) :699-710

[6] Widespread Rewiring of Genetic Networks upon Cancer Signaling Pathway Activation [J].

Billmann, Maximilian ;

Chaudhary, Varun ;

ElMaghraby, Mostafa F. ;

Fischer, Bernd ;

Boutros, Michael .

CELL SYSTEMS, 2018, 6 (01) :52-+

[7] Dual gene activation and knockout screen reveals directional dependencies in genetic networks [J].

Boettcher, Michael ;

Tian, Ruilin ;

Blau, James A. ;

Markegard, Evan ;

Wagner, Ryan T. ;

Wu, David ;

Mo, Xiulei ;

Biton, Anne ;

Zaitlen, Noah ;

Fu, Haian ;

McCormick, Frank ;

Kampmann, Martin ;

McManus, Michael T. .

NATURE BIOTECHNOLOGY, 2018, 36 (02) :170-+

[8] Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR [J].

Boettcher, Michael ;

McManus, Michael T. .

MOLECULAR CELL, 2015, 58 (04) :575-585

[9] A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies [J].

Breslow, David K. ;

Hoogendoorn, Sascha ;

Kopp, Adam R. ;

Morgens, David W. ;

Vu, Brandon K. ;

Kennedy, Margaret C. ;

Han, Kyuho ;

Li, Amy ;

Hess, Gaelen T. ;

Bassik, Michael C. ;

Chen, James K. ;

Nachury, Maxence V. .

NATURE GENETICS, 2018, 50 (03) :460-471

[10] CRISPR-Cas9 screen reveals a MYCN-amplified neuroblastoma dependency on EZH2 [J].

Chen, Liying ;

Alexe, Gabriela ;

Dharia, Neekesh V. ;

Ross, Linda ;

Iniguez, Amanda Balboni ;

Conway, Amy Saur ;

Wang, Emily Jue ;

Veschi, Veronica ;

Lam, Norris ;

Qi, Jun ;

Gustafson, W. Clay ;

Nasholm, Nicole ;

Vazquez, Francisca ;

Weir, Barbara A. ;

Cowley, Glenn S. ;

Ali, Levi D. ;

Pantel, Sasha ;

Jiang, Guozhi ;

Harrington, William F. ;

Lee, Yenarae ;

Goodale, Amy ;

Lubonja, Rakela ;

Krill-Burger, John M. ;

Meyers, Robin M. ;

Tsherniak, Aviad ;

Root, David E. ;

Bradner, James E. ;

Golub, Todd R. ;

Roberts, Charles W. M. ;

Hahn, William C. ;

Weiss, William A. ;

Thiele, Carol J. ;

Stegmaier, Kimberly .

JOURNAL OF CLINICAL INVESTIGATION, 2018, 128 (01) :446-462

← 1 2 3 4 5 6 7 8 →