CHARACTERIZATION OF MICROSATELLITE MARKERS IN THE AFRICAN TROPICAL TREE SPECIES GUIBOURTIA EHIE (FABACEAE, DETARIOIDEAE)

被引：1

作者：

Tosso, Felicien ^{[1
,2
]}

Doucet, Jean-Louis ^{[1
]}

Migliore, Jeremy ^{[2
]}

Dainou, Kasso ^{[1
,3
,4
,5
]}

Kaymak, Esra ^{[2
]}

Kameni, Franck S. Monthe ^{[2
]}

Hardy, Olivier J. ^{[2
]}

机构：

[1] Univ Liege, Gembloux Agrobio Tech, TERRA Teaching & Res Ctr, Cent African Forests, Passage Deportes 2, B-5030 Gembloux, Belgium

[2] Univ Libre Bruxelles, Fac Sci, Evolutionary Biol & Ecol Unit, CP 160-12,Av FD Roosevelt 50, B-1050 Brussels, Belgium

[3] Nat Asbl, Rue Prov 62, B-1301 Wavre, Belgium

[4] Univ Liege, Gembloux Agrobio Tech, BIOSE Dept, Management Forest Resources, B-5030 Gembloux, Belgium

[5] Univ Agr Ketou, BP 43 Ketou, Ketou, Benin

来源：

APPLICATIONS IN PLANT SCIENCES | 2017年 / 5卷 / 07期

关键词：

Fabaceae; Guibourtia ehie; microsatellites; next-generation sequencing; COMPUTER-PROGRAM;

D O I：

10.3732/apps.1700023

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise of the study: Microsatellite primers (simple sequence repeats [SSRs]) were developed in Guibourtia ehie (Fabaceae, Detarioideae) to study population genetic structure and the history of African vegetation. Methods and Results: We isolated 18 polymorphic SSRs from a nonenriched genomic library. This set of primer pairs was tested on four populations, and the results showed two to 16 alleles per locus with mean observed and expected heterozygosities of 0.27 0.05 and 0.57 +/- 0.05, respectively. Cross-amplification tests in 13 congeneric species were successful for the four taxa belonging to the subgenus Gorskia. Conclusions: This set of microsatellite markers will be useful to investigate the phylogeography and population genetics of G. ehie, a key representative of African semideciduous moist forests.

引用

页数：5

共 13 条

[1] [Anonymous], 1995, TROPICAL FORESTRY PA
[2] Simultaneous Estimation of Null Alleles and Inbreeding Coefficients
Chybicki, Igor J.
Burczyk, Jaroslaw
[J]. JOURNAL OF HEREDITY, 2009, 100 (01) : 106 - 113
[3] Construction of a SSH library of Aegiceras corniculatum under salt stress and expression analysis of four transcripts
Fu, XH
Huang, YL
Deng, SL
Zhou, RC
Yang, GL
Ni, XW
Li, WJ
Shi, SH
[J]. PLANT SCIENCE, 2005, 169 (01) : 147 - 154
[4] SPAGEDi: a versatile computer program to analyse spatial genetic structure at the individual or population levels
Hardy, OJ
Vekemans, X
[J]. MOLECULAR ECOLOGY NOTES, 2002, 2 (04): : 618 - 620
[5] Multiplex Manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR
Holleley, Clare E.
Geerts, Paul G.
[J]. BIOTECHNIQUES, 2009, 46 (07) : 511 - +
[6] Lemmens R. H. M. J., 2008, BOIS DOEUVRE, V2
[7] Cost-effective enrichment hybridization capture of chloroplast genomes at deep multiplexing levels for population genetics and phylogeography studies
Mariac, Cedric
Scarcelli, Nora
Pouzadou, Juliette
Barnaud, Adeline
Billot, Claire
Faye, Adama
Kougbeadjo, Ayite
Maillol, Vincent
Martin, Guillaume
Sabot, Francois
Santoni, Sylvain
Vigouroux, Yves
Couvreur, Thomas L. P.
[J]. MOLECULAR ECOLOGY RESOURCES, 2014, 14 (06) : 1103 - 1113
[8] PANDAseq: PAired-eND Assembler for Illumina sequences
Masella, Andre P.
Bartram, Andrea K.
Truszkowski, Jakub M.
Brown, Daniel G.
Neufeld, Josh D.
[J]. BMC BIOINFORMATICS, 2012, 13
[9] QDD version 3.1: a user-friendly computer program for microsatellite selection and primer design revisited: experimental validation of variables determining genotyping success rate
Meglecz, Emese
Pech, Nicolas
Gilles, Andre
Dubut, Vincent
Hingamp, Pascal
Trilles, Aurelie
Grenier, Remi
Martin, Jean-Francois
[J]. MOLECULAR ECOLOGY RESOURCES, 2014, 14 (06) : 1302 - 1313
[10] DEVELOPMENT AND CHARACTERIZATION OF MICROSATELLITE LOCI IN PERICOPSIS ELATA (FABACEAE) USING A COST-EFFICIENT APPROACH
Micheneau, Claire
Dauby, Gilles
Bourland, Nils
Doucet, Jean-Louis
Hardy, Olivier J.
[J]. AMERICAN JOURNAL OF BOTANY, 2011, 98 (10) : E268 - E270

← 1 2 →