Investigation of voltammetric enzyme-linked immunoassay based on new system of OT-H2O2-HRP

被引：0

作者：

Jiao, K ^{[1
]}

Zhang, SS ^{[1
]}

Wei, L ^{[1
]}

Liu, CF ^{[1
]}

机构：

[1] Qingdao Inst Chem Technol, Dept Appl Chem, Qingdao 266042, Peoples R China

来源：

ACTA CHIMICA SINICA | 1997年 / 55卷 / 11期

关键词：

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A voltammetric enzyme-linked immunoassay based on a new system of o-tilidine (OT)-H2O2-horseradish peroxidase (HRP) has firstly been developed. In this method, the enzyme-catalyzing reaction of H2O2 oxidizing OT couples the electro-reduction reaction of the oxidizing product of OT, which produces a sensitive voltammetric wave at potential of -0.58V (vs. SCE) in Britton-Robinson buffer solution. When it is used in the detection of free HRP and labelled HRP, the sensitivity is nearly two and four-order higher than that of classical ELISA method, respectively. The detection limit to HRP is 1.8X10(-12) g/mL and the linear range 5.0X10(-12)similar to 1.0X10(-8) g/mL. The mechanism of the voltammetric enzyme-linked immunoassay system has also been studied in detail.

引用

页码：1121 / 1129

页数：9

共 8 条

[1] EVERSE J, 1991, PEROXIDASE CHEM BIOL, V2, P5
[2] FLORENCE TM, 1974, J ELECTROANAL CHEM, V52, P115, DOI 10.1016/S0022-0728(74)80107-5
[3] DETERMINATION OF MOLYBDENUM IN SOILS AND PLANTS USING THE POLAROGRAPHIC ADSORPTIVE COMPLEX CATALYTIC WAVE OF MO(VI)-CUPFERRON
JIAO, K
JIN, W
METZNER, H
[J]. ANALYTICA CHIMICA ACTA, 1992, 260 (01) : 35 - 43
[4] LIU CF, 1990, ANAL CHIM ACTA, V238, P367
[5] LOWIS GP, 1965, J CLIN PATHOL, V2, P235
[6] MATRKA M, 1971, CHEM PRUM, V21, P14
[7] MOHR P, 1983, BIOMED BIOCHIM ACTA, V6, P663
[8] MONGET D, 1978, AM BIOL CLIN, V6, P527

← 1 →