High-resolution quantitative profiling of tRNA abundance and modification status in eukaryotes by mim-tRNAseq

被引：146

作者：

Behrens, Andrew ^{[1
]}

Rodschinka, Geraldine ^{[1
]}

Nedialkova, Danny D. ^{[1
,2
]}

机构：

[1] Max Planck Inst Biochem, Mech Prot Biogenesis, D-82152 Martinsried, Germany

[2] Tech Univ Munich, Dept Chem, D-85748 Garching, Germany

来源：

MOLECULAR CELL | 2021年 / 81卷 / 08期

基金：

欧洲研究理事会; 英国惠康基金;

关键词：

READ ALIGNMENT; MESSENGER-RNA; REVEALS; COMPLEX; SEQ; IDENTIFICATION; MITOCHONDRIAL; NUCLEOTIDES; RECOGNITION; LANDSCAPE;

D O I：

10.1016/j.molcel.2021.01.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Measurements of cellular tRNA abundance are hampered by pervasive blocks to cDNA synthesis at modified nucleosides and the extensive similarity among tRNA genes. We overcome these limitations with modification-induced misincorporation tRNA sequencing (mim-tRNAseq), which combines a workflow for full-length cDNA library construction from endogenously modified tRNA with a comprehensive and user-friendly computational analysis toolkit. Our method accurately captures tRNA abundance and modification status in yeast, fly, and human cells and is applicable to any organism with a known genome. We applied mim-tRNAseq to discover a dramatic heterogeneity of tRNA isodecoder pools among diverse human cell lines and a surprising interdependence of modifications at distinct sites within the same tRNA transcript.

引用

页码：1802 / +

页数：21

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