Sorting a Staphylococcus aureus phage display library against ex vivo biomaterial

被引：8
|
作者：
Bjerketorp, J [1 ]
Rosander, A [1 ]
Nilsson, M [1 ]
Jacobsson, K [1 ]
Frykberg, L [1 ]
机构：
[1] Swedish Univ Agr Sci, Dept Microbiol, SE-75007 Uppsala, Sweden
来源：
JOURNAL OF MEDICAL MICROBIOLOGY | 2004年 / 53卷 / 10期
关键词：
D O I：
10.1099/jmm.0.45638-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A phage display library made from Staphylococcus aureus DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50% of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and beta(2)-glycoprotein I (beta(2)-GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, beta(2)-GPI was also detected on all tested CVCs.
引用
收藏
页码：945 / 951
页数：7
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