Sorting a Staphylococcus aureus phage display library against ex vivo biomaterial

被引：8

作者：

Bjerketorp, J ^{[1
]}

Rosander, A ^{[1
]}

Nilsson, M ^{[1
]}

Jacobsson, K ^{[1
]}

Frykberg, L ^{[1
]}

机构：

[1] Swedish Univ Agr Sci, Dept Microbiol, SE-75007 Uppsala, Sweden

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 2004年 / 53卷 / 10期

关键词：

D O I：

10.1099/jmm.0.45638-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A phage display library made from Staphylococcus aureus DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50% of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and beta(2)-glycoprotein I (beta(2)-GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, beta(2)-GPI was also detected on all tested CVCs.

引用

页码：945 / 951

页数：7

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