Suppressive effect of dexamethasone on TIMP-1 production involves murine osteoblastic MC3T3-E1 cell apoptosis

被引:23
|
作者
Xie, Hui [1 ]
Tang, Ling-Li [2 ]
Luo, Xiang-Hang [1 ]
Wu, Xi-Yu [1 ]
Wu, Xian-Ping [1 ]
Zhou, Hou-De [1 ]
Yuan, Ling-Qing [1 ]
Liao, Er-Yuan [1 ]
机构
[1] Cent S Univ, Xiangya Hosp 2, Inst Endocrinol & Metab, Changsha 410011, Hunan, Peoples R China
[2] Cent S Univ, Xiangya Hosp 2, Dept Clin Lab, Changsha 410011, Hunan, Peoples R China
关键词
Dexamethasone; Tissue inhibitor of metalloproteinase-1; Apoptosis; Osteoblast; GLUCOCORTICOID-INDUCED OSTEOPOROSIS; TISSUE INHIBITOR; IN-VITRO; STEROID-HORMONES; BONE-FORMATION; B-CELLS; METALLOPROTEINASE-1; EXPRESSION; DIFFERENTIATION; PROLIFERATION;
D O I
10.1007/s00726-009-0325-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
High dose glucocorticoid (GC) treatment induces osteoporosis partly via increasing osteoblast apoptosis. However, the mechanism of GC-induced apoptosis has not been fully elucidated. Osteoblast-derived tissue inhibitor of metalloproteinase-1 (TIMP-1) was recently reported to be involved in bone metabolism. Our previous study demonstrated that TIMP-1 suppressed apoptosis of the mouse bone marrow stromal cell line MBA-1 (pre-osteoblast) induced by serum deprivation. Therefore, we tested the effect of the GC dexamethasone (Dex) on TIMP-1 production in murine osteoblastic MC3T3-E1 cells and further determined whether this action is associated with Dex-induced osteoblast apoptosis. Dex decreased TIMP-1 production in MC3T3-E1 cells, and this effect was blocked by the glucocorticoid receptor (GR) antagonists, RU486 and RU40555. Recombinant TIMP-1 protein reduced caspase-3 activation and apoptosis induced by Dex in MC3T3-E1 cells. In addition, the pro-apoptotic effect of the Dex was augmented by suppression of TIMP-1 with siRNA. Furthermore, mutant TIMP-1, which has no inhibitory effects on MMPs, yet protects MC3T3-E1 cells against Dex-induced apoptosis. Our study demonstrates that Dex suppresses TIMP-1 production in osteoblasts through GR, and this effect is associated with its induction of osteoblast apoptosis. The anti-apoptotic action of TIMP-1 is independent of its inhibitory effects on MMPs activities. The decrease in TIMP-1 production caused by Dex may contribute to the mechanisms of Dex-induced bone loss.
引用
收藏
页码:1145 / 1153
页数:9
相关论文
共 50 条
  • [31] Sunflower seed extract enhances the differentiation of osteoblastic MC3T3-E1 cells
    Choi, Eun Mi
    FOOD AND AGRICULTURAL IMMUNOLOGY, 2014, 25 (01) : 9 - 19
  • [32] Quantitative proteomic analysis of dexamethasone-induced effects on osteoblast differentiation, proliferation, and apoptosis in MC3T3-E1 cells using SILAC
    Hong, D.
    Chen, H. -X.
    Yu, H. -Q.
    Wang, C.
    Deng, H. -T.
    Lian, Q. -Q.
    Ge, R. -S.
    OSTEOPOROSIS INTERNATIONAL, 2011, 22 (07) : 2175 - 2186
  • [33] Effects of Scytosiphon lomentaria on osteoblastic proliferation and differentiation of MC3T3-E1 cells
    Park, Mi Hwa
    Kim, Seoyeon
    Cheon, Jihyeon
    Lee, Juyeong
    Kim, Bo Kyung
    Lee, Sang-Hyeon
    Kong, Changsuk
    Kim, Yuck Yong
    Kim, Mihyang
    NUTRITION RESEARCH AND PRACTICE, 2016, 10 (02) : 148 - 153
  • [34] Effect of Ethanol Extract Concentrations of Hei Chou on Calcification of MC3T3-E1 cell
    Ahn, Kwang-Sik
    Lee, Young-Hoon
    Kwon, Ha-Jeong
    Park, Yong-Duk
    2010 3RD INTERNATIONAL CONFERENCE ON BIOMEDICAL ENGINEERING AND INFORMATICS (BMEI 2010), VOLS 1-7, 2010, : 1936 - 1939
  • [35] Liraglutide attenuates the osteoblastic differentiation of MC3T3-E1 cells by modulating AMPK/mTOR signaling
    Hu, Xiong-Ke
    Yin, Xin-Hua
    Zhang, Hong-Qi
    Guo, Chao-Feng
    Tang, Ming-Xing
    MOLECULAR MEDICINE REPORTS, 2016, 14 (04) : 3662 - 3668
  • [36] Dexamethasone induces sodium-dependant vitamin C transporter in a mouse osteoblastic cell line MC3T3-E1
    Fujita, I
    Hirano, J
    Itoh, N
    Nakanishi, T
    Tanaka, K
    BRITISH JOURNAL OF NUTRITION, 2001, 86 (02) : 145 - 149
  • [37] Stimulation of Osteoblastic Differentiation and Mineralization in MC3T3-E1 Cells by Yeast Hydrolysate
    Lee, Hyun-Sun
    Jung, Eun-Young
    Bae, Song Hwan
    Kwon, Ki Han
    Kim, Jin-Man
    Suh, Hyung Joo
    PHYTOTHERAPY RESEARCH, 2011, 25 (05) : 716 - 723
  • [38] Promoting Effect of Pinostrobin on the Proliferation, Differentiation, and Mineralization of Murine Pre-osteoblastic MC3T3-E1 Cells
    Gu, Chengbo
    Fu, Linan
    Yuan, Xiaohan
    Liu, Zhiguo
    MOLECULES, 2017, 22 (10)
  • [39] Evaluation of MC3T3-E1 Cell Osteogenesis in Different Cell Culture Media
    Izumiya, Makoto
    Haniu, Miyu
    Ueda, Katsuya
    Ishida, Haruka
    Ma, Chuang
    Ideta, Hirokazu
    Sobajima, Atsushi
    Ueshiba, Koki
    Uemura, Takeshi
    Saito, Naoto
    Haniu, Hisao
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (14)
  • [40] Effect of acetaminophen on osteoblastic differentiation and migration of MC3T3-E1 cells
    Yoshihiro Nakatsu
    Fumio Nakagawa
    Sen Higashi
    Tomoko Ohsumi
    Shunji Shiiba
    Seiji Watanabe
    Hiroshi Takeuchi
    Pharmacological Reports, 2018, 70 : 29 - 36