Riboswitches are genetic regulatory elements found in the 50 untranslated region of messenger RNA that act in the absence of protein cofactors(1,2). They are broadly distributed across bacteria and account for the regulation of more than 2% of all genes in Bacillus subtilis, underscoring their importance in the control of cellular metabolism(3). The 5' untranslated region of many mRNAs of genes involved in purine metabolism and transport contain a guanine-responsive riboswitch that directly binds guanine, hypoxanthine or xanthine to terminate transcription(3,4). Here we report the crystal structure at 1.95 Angstrom resolution of the purine-binding domain of the guanine riboswitch from the xpt-pbuX operon of B. subtilis bound to hypoxanthine, a prevalent metabolite in the bacterial purine salvage pathway. This structure reveals a complex RNA fold involving several phylogenetically conserved nucleotides that create a binding pocket that almost completely envelops the ligand. Hypoxanthine functions to stabilize this structure and to promote the formation of a downstream transcriptional terminator element, thereby providing a mechanism for directly repressing gene expression in response to an increase in intracellular concentrations of metabolite.
机构:Univ Calif Santa Cruz, Sinsheimer Labs, Dept Biol, Santa Cruz, CA 95064 USA
Baugh, C
;
Grate, D
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机构:Univ Calif Santa Cruz, Sinsheimer Labs, Dept Biol, Santa Cruz, CA 95064 USA
Grate, D
;
Wilson, C
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Univ Calif Santa Cruz, Sinsheimer Labs, Dept Biol, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Sinsheimer Labs, Dept Biol, Santa Cruz, CA 95064 USA
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MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USAMEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USA
Fan, P
;
Suri, AK
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MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USAMEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USA
Suri, AK
;
Fiala, R
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MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USAMEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USA
Fiala, R
;
Live, D
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MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USAMEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USA
Live, D
;
Patel, DJ
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机构:
MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USAMEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USA
机构:Univ Calif Santa Cruz, Sinsheimer Labs, Dept Biol, Santa Cruz, CA 95064 USA
Baugh, C
;
Grate, D
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif Santa Cruz, Sinsheimer Labs, Dept Biol, Santa Cruz, CA 95064 USA
Grate, D
;
Wilson, C
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Santa Cruz, Sinsheimer Labs, Dept Biol, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Sinsheimer Labs, Dept Biol, Santa Cruz, CA 95064 USA
机构:
MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USAMEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USA
Fan, P
;
Suri, AK
论文数: 0引用数: 0
h-index: 0
机构:
MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USAMEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USA
Suri, AK
;
Fiala, R
论文数: 0引用数: 0
h-index: 0
机构:
MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USAMEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USA
Fiala, R
;
Live, D
论文数: 0引用数: 0
h-index: 0
机构:
MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USAMEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USA
Live, D
;
Patel, DJ
论文数: 0引用数: 0
h-index: 0
机构:
MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USAMEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USA