Speciation of selenium in selenium-enriched shiitake mushroom, Lentinula edodes

被引:85
作者
Ogra, Y [1 ]
Ishiwata, K
Encinar, JR
Lobinski, R
Suzuki, KT
机构
[1] Chiba Univ, Grad Sch Pharmaceut Sci, Chuo Ku, Chiba 2608675, Japan
[2] CNRS, UMR 5034, F-64053 Pau, France
[3] Warsaw Univ Technol, Dept Chem, PL-00664 Warsaw, Poland
基金
日本学术振兴会;
关键词
selenium; speciation; Lentinula edodes; ICP MS; ESI MS; selenomethionine;
D O I
10.1007/s00216-004-2670-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The major selenium compound in an aqueous extract of the most popular mushroom in Eastern Asian countries, shiitake (Lentinula edodes), fortified with selenium (Se) was identified by means of hyphenated techniques, i.e. HPLC-inductively coupled argon plasma mass spectrometry and HPLC-electrospray ionization mass spectrometry (HPLC-ICP MS and HPLC-ESI MS). Sixty-eight per cent of the total Se in the selenized shiitake was extracted with water, and 49.8% of the Se in the water extract was eluted in the high molecular mass fraction (> 40,000 kDa) before incubation at 37 degreesC. After incubation, 40.6% of the Se in the water extract was eluted in a lower molecular mass fraction and the Se eluted in the high molecular mass fraction had decreased to 14.0%, suggesting that the major selenium compound in the water extract was initially in a form bound to macromolecule(s) and was then enzymatically liberated from the macromolecule(s). The retention time of the liberated selenium compound in HPLC-ICP MS matched that of selenomethionine (SeMet), and the masses of molecular and fragment ions detected by HPLC-ESI MS also suggested that the selenium compound was SeMet. The selenized shiitake accumulated Se as SeMet, and SeMet might be bound to the water extractable high molecular mass protein(s).
引用
收藏
页码:861 / 866
页数:6
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