Effect of some growth regulators on "in vitro" culture of two Vitis vinifera L. cultivars

Shoot apical meristems were used for in vitro regeneration in 2 grape cultivars - "Soltanin" and "Sahebi". Meristems were taken from 10 cm length shoots. The culture media used in this study was MS (Murashige & Skoog, 1962), with hormonal supplements consisting of: A (1 mgL(-1) BA), B (1.5 mgL(-1) BA), C (1 mgL(-1) IBA+ 1.5 mgL(-1) BA) and D (1 mgL(-1) TDZ). Results indicated that B and C variants of culture media produced the highest average number of shoots per cultured apex (3.8-5.4). Consequently, the shoots were twice subcultured on a medium variant supplemented with 1 mg/L TDZ+ 1.5 mg/L GA. The "in vitro" derived shoots were pretreated with 1 mgL(-1) IBA, and then directly potted, which caused significant enhancement in root number per shoot. The good rooting achieved under "in vivo" conditions resulted in the reduction of the time spent for vitroplant acclimatization compared with "in vitro" rooting and, therefore, the time needed for plantlet regeneration was shortened. There was no significant difference between the two cultivars regarding the measured traits.