Analyzing the Interaction between Two Different Types of Nanoparticles and Serum Albumin

被引：16

作者：

Cristian, Roxana E. ^{[1
]}

Mohammad, Israa J. ^{[1
]}

Mernea, Maria ^{[2
]}

Sbarcea, Beatrice G. ^{[3
]}

Trica, Bogdan ^{[4
]}

Stan, Miruna S. ^{[1
,5
]}

Dinischiotu, Anca ^{[1
]}

机构：

[1] Univ Bucharest, Dept Biochem & Mol Biol, Fac Biol, 91-95 Spl Independentei, Bucharest 050095, Romania

[2] Univ Bucharest, Dept Anat Anim Physiol & Biophys, Fac Biol, 91-95 Spl Independentei, Bucharest 050095, Romania

[3] Natl Inst Res & Dev Elect Engn ICPE CA, Mat Characterizat Dept, 313 Splaiul Unirii, Bucharest 030138, Romania

[4] Natl Inst Res & Dev Chem & Petrochem INCDCP ICECH, 202 Spl Independentei, Bucharest 060021, Romania

[5] Univ Politehn Bucuresti, Fac Appl Chem & Mat Sci, Dept Sci & Engn Oxide Mat & Nanomat, 1-7 Gheorghe Polizu Str, Bucharest 011061, Romania

来源：

MATERIALS | 2019年 / 12卷 / 19期

关键词：

biocorona; nanoparticles; serum albumin; silica; magnetite; titanium dioxide; MESOPOROUS SILICA NANOPARTICLES; PLASMA-PROTEIN ADSORPTION; TOXICITY; SIZE; BIOCOMPATIBILITY; AGGLOMERATION; STABILITY; CORONA;

D O I：

10.3390/ma12193183

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Two different types of nanoparticles (silicon dioxide and titanium dioxide) were selected within this study in order to analyze the interaction with bovine and human serum albumin. These particles were characterized by transmission and scanning electron microscopy (TEM and SEM), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDXS). In addition, the hydrodynamic size and the zeta potential were measured for all these nanoparticles. The serum proteins were incubated with the nanoparticles for up to one hour, and the albumin adsorption on the particle surface was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The effect induced on the secondary structure of proteins was analyzed by Fourier transform infrared spectroscopy (FTIR). The results showed that albumin adsorbed on the surface of both types of nanoparticles, but in different quantities. In addition, we noticed different changes in the structure of albumin depending on the physicochemical properties of each type of particle tested. In conclusion, our study provides a comparative analysis between the different characteristics of nanoparticles and the protein corona formed on the particle surface and effects induced on protein structure in order to direct the development of "safe-by-design" nanoparticles, as their demands for research and applications continue to increase.

引用

页数：16

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