N-glycans harboring the Lewis a epitope are expressed at the surface of plant cells

被引:161
作者
Fitchette-Lainé, AC
Gomord, V
Cabanes, M
Michalski, JC
Saint Macary, M
Foucher, B
Cavelier, B
Hawes, C
Lerouge, P
Faye, L
机构
[1] Univ Rouen, Fac Sci, IFRMP 23, ESA CNRS 6037, F-76821 Mt St Aignan, France
[2] Univ Sci & Tech Lille Flandres Artois, UMR CNRS 111, Lab Chim Biol C9, F-59655 Villeneuve Dascq, France
[3] Etab Transfus Sanguine & Genet Humaine Haute Norm, F-76232 Bois Guillaume, France
[4] Oxford Brookes Univ, Res Sch Biol & Mol Sci, Oxford OX3 0BP, England
关键词
D O I
10.1046/j.1365-313x.1997.12061411.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In plants, N-linked glycans are processed in the Golgi apparatus to complex-type N-glycans of limited size containing a beta(1,2)-xylose and/or an alpha(1,3)-fucose residue. Larger mono-and bi-antennary N-linked complex glycans have not often been described. This study has re-examined the structure of such plant N-linked glycans, and, through both immunological and structural data, it is shown that the antennae are composed of Lewis a (Le(a)) antigens, comprising the carbohydrate sequence Gal beta 1-3[Fuc alpha 1-4]GlcNAc. Furthermore, a fucosyltransferase activity involved in the biosynthesis of this antigen was detected in sycamore cells. This is the first characterization in plants of a Lewis antigen that is usually found on cell-surface glycoconjugates in mammals and involved in recognition and adhesion processes. Le(a)-containing N-linked glycans are widely distributed in plants and highly expressed at the cell surface, which may suggest a putative function in cell/cell communication.
引用
收藏
页码:1411 / 1417
页数:7
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