Assessment of the triacylglycerol fraction of olive oil by 1D-NMR spectroscopy: exploring the usefulness of DEPT tool on the peak assignments of 13C NMR spectra

被引:5
作者
Garcia, Raquel [1 ]
Pires, Arona [2 ]
Martins, Nuno [3 ]
Carvalho, Teresa [4 ]
Burke, Anthony J. [2 ,5 ]
Cabrita, Maria Joao [6 ]
机构
[1] Univ Evora, Nucleo Mitra, IIFA, ICAAM Inst Ciencias Agr & Ambientais Mediterran, Ap 94, P-7006554 Evora, Portugal
[2] Univ Evora, Ctr Quim Evora, Rua Romao Ramalho 59, P-7000 Evora, Portugal
[3] Univ Avignon, Aix Marseille Univ, CNRS, IRD,IMBE, Marseille, France
[4] Inst Nacl Invest Agr & Vet INIAV IP, Elvas, Portugal
[5] Univ Evora, Escola Ciencias & Tecnol, Dept Quim, Rua Romao Ramalho 59, P-7000 Evora, Portugal
[6] Univ Evora, ICAAM Inst Ciencias Agr & Ambientais Mediterran, Escola Ciencias & Tecnol, Dept Fitotecnia, Campus Mitra, P-7000083 Evora, Portugal
关键词
Olive oil; Fatty acid; Triacylglycerol fraction; Nuclear magnetic resonance (NMR); NUCLEAR-MAGNETIC-RESONANCE; GEOGRAPHICAL ORIGIN; CLASSIFICATION; COMPONENTS; QUALITY; H-1; GC;
D O I
10.1007/s00217-019-03359-0
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Nuclear magnetic resonance (NMR) spectroscopy has been extensively used to date for the analysis of olive oil samples and constitutes a valuable tool particularly for the study of the triacylglycerol fraction, which is the major fraction of olive oils. In this report, we demonstrate the usefulness of one-dimensional NMR spectroscopy (H-1 and C-13 NMR) for the non-destructive analysis of the triacylglycerol fraction of olive oils. To achieve this goal, C-13 spectral editing technique known as distortionless enhancement by polarization transfer (DEPT), namely C-13 NMR DEPT 45, C-13 NMR DEPT 90, C-13 NMR DEPT 135 was used for the analysis of the triacylglycerol fraction of the olive oil sample owing to the simplification on peak assignments of C-13 spectra. The suitability and usefulness of those DEPT experiments using different angle values in only one pulse sequence will be explored on the interpretation of C-13 NMR spectra. In overall, DEPT tool improves the sensitivity of the analysis due to the enhancement in the signal-to-noise ratio, which is important for this type of analysis contributing to the characterization of olive oils.
引用
收藏
页码:2479 / 2488
页数:10
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