High Expression of CD200 and CD200R1 Distinguishes Stem and Progenitor Cell Populations within Mammary Repopulating Units

被引：11

作者：

Rauner, Gat ^{[1
,2
,4
]}

Kudinov, Tania ^{[1
,2
]}

Gilad, Shlomit ^{[3
]}

Hornung, Gil ^{[3
]}

Barash, Itamar ^{[1
]}

机构：

[1] ARO, Volcani Ctr, Inst Anim Sci, IL-50250 Bet Dagan, Israel

[2] Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, IL-7610001 Rehovot, Israel

[3] Weizmann Inst Sci, Nancy & Stephen Grand Israel Natl Ctr Personalize, IL-7610001 Rehovot, Israel

[4] Cornell Univ, Coll Vet Med, Baker Inst Anim Hlth, Ithaca, NY 14850 USA

来源：

STEM CELL REPORTS | 2018年 / 11卷 / 01期

基金：

以色列科学基金会;

关键词：

BREAST-CANCER; GLAND DEVELOPMENT; COMPLEMENT; PATHWAY; DIFFERENTIATION; IDENTIFICATION; PROMOTES; SIGNALS; DISEASE; GROWTH;

D O I：

10.1016/j.stemcr.2018.05.013

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Aiming to unravel the top of the mammary epithelial cell hierarchy, a subset of the CD49f(high) CD24(med) mammary repopulating units (MRUs) was identified by flow cytometry, expressing high levels of CD200 and its receptor CD200R1. These MRUCD200/CD200R1 repopulated a larger area of de-epithelized mammary fat pads than the rest of the MRUs, termed MRUnotCD200/CD200R1. MRUCD200/CD200R1 maintained a much lower number of divergently defined, highly expressed genes and pathways that support better cell growth, development, differentiation, and progenitor activity than their MRUnotCD200/CD200R1 counterparts. A defined profile of hierarchically associated genes supporting a single-lineage hypothesis was confirmed by in vitro mammosphere analysis that assembled 114 genes with decreased expression from MRUCD200/CD200R1 via MRUnotCD200/CD200R1 toward CD200(+)CD200R1(-) and CD200R1(+)CD200(-) cells. About 40% of these genes were shared by a previously published database of upregulated genes in mammary/breast stem cells and may represent the core genes involved in mammary stemness.

引用

页码：288 / 302

页数：15

共 63 条

[1] HTSeq-a Python']Python framework to work with high-throughput sequencing data [J].