Polymorphic membrane protein 20G: A promising diagnostic biomarker for specific detection of Chlamydia psittaci infection

被引：8

作者：

Cui, Lei ^{[1
]}

Qu, Guanggang ^{[2
]}

Chen, Yi ^{[3
]}

Wu, Yuexing ^{[2
]}

Wang, Changjiang ^{[2
]}

Cheng, He ^{[3
]}

Chen, Jianlin ^{[1
]}

机构：

[1] Cent South Univ, Xiangya Hosp 2, Reprod Med Ctr, Dept Obstet & Gynecol, Changsha, Hunan, Peoples R China

[2] Shandong Binzhou Anim Sci & Vet Med Acad, Binzhou, Shandong, Peoples R China

[3] China Agr Univ, Coll Vet Med, Key Lab Anim Epidemiol & Zoonosis, Minist Agr & Rural Affairs, Beijing, Peoples R China

来源：

MICROBIAL PATHOGENESIS | 2021年 / 155卷

基金：

中国国家自然科学基金; 北京市自然科学基金;

关键词：

Chlamydia psittaci; Pmp20G-N; Indirect-ELISA; III SECRETION SYSTEM; OUTER-MEMBRANE; CHLAMYDOPHILA-ABORTUS; IMMUNE-RESPONSES; IN-OVINE; TRACHOMATIS; ANTIBODIES; EXPRESSION; SEQUENCES; SURFACE;

D O I：

10.1016/j.micpath.2021.104882

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Psittacosis is a zoonotic disease caused by Chlamydia psittaci (C. psittaci), leading to high risk for animal industry and human health. Lack of reliable commercial kits and effective vaccines is hampering control of C. psittaci infection. Polymorphic outer membrane protein Gs (PmpGs) are enriched in diverse C. psittaci, and its role are unclear during C. psittaci infection. In the present study, pmp20G gene was cloned into pET-28a vector and then the constructed plasmid was transferred into Escherichia coli Rossetta (DE3). After denaturation and renaturation, the recombinant Pmp20G-N was identified by SDS-PAGE and Western blot. Afterwards Pmp20G-N was used as the coating antigen to develop an indirect ELISA (I-ELISA) assay. Both the specificity and sensitivity of Pmp20GN ELISA were 100%, while the MOMP-ELISA had 93.65% sensitivity and 98.94% specificity, respectively. The concordance between MOMP-ELISA and Pmp20G-N ELISA assay was 98.1%. Hence, Pmp20G-N ELISA has the potential to be a diagnostic antigen for detection C. psittaci antibody. However, further studies are needed to be done for differentiating C. psittaci from Chlamydia spp. and other C.psittaci-specific serovars using Pmp20G-N ELISA.

引用

页数：7

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