ITS-RFLP and ITS sequence analysis of a foliar endophytic Phyllosticta from different tropical trees

被引：48
作者：
Pandey, AK
Reddy, MS [1 ]
Suryanarayanan, TS
机构：
[1] Thapar Inst Engn & Technol, Dept Biotechnol, Patiala 147001, Punjab, India
[2] Vivekananda Coll, Ramakrishna Mission, Post Grad & Res Dept Bot, Madras 600004, Tamil Nadu, India
来源：
MYCOLOGICAL RESEARCH | 2003年 / 107卷
关键词：
D O I：
10.1017/S0953756203007494
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Different isolates of a foliar endophytic species of Phyllosticta were isolated from different tropical tree species in India to examine genetic variation among the isolates. Internal transcribed spacer-restriction fragment length polymorphism (ITS-RFLP) analysis did not detect any variation among the isolates, suggesting that they all belong to the same species. Sequence data of the ITS region of ribosomal DNA (ITS1 and ITS2, including 5.8S rDNA) supported the identity of the present fungal isolates as P. capitalensis. These results show that P. copitalensis (teleomorph Guignardia endophyllicola?) is an ubiquitous foliar endophyte that can infect tree hosts from different families and habitats.
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页码：439 / 444
页数：6
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