Interlaboratory evaluation of the Etest® for antifungal susceptibility testing of dermatophytes

被引：23

作者：

Fernández-Torres, B

Carrillo-Muñoz, A

Ortoneda, M

Pujol, I

Pastor, FJ

Guarro, J

机构：

[1] Univ Rovira & Virgili, Inst Estudis Avancats, Fac Med, Unitat Microbiol, E-43201 Reus, Tarragona, Spain

[2] Asesoria Cient & Invest Aplicada, Dept Microbiol, Barcelona 08080, Spain

[3] Hosp Univ St Joan Reus, Microbiol Lab, Tarragona, Spain

来源：

MEDICAL MYCOLOGY | 2003年 / 41卷 / 02期

关键词：

broth microdilution; dermatophytes; Etest (R); in vitro susceptibility;

D O I：

10.1080/714043907

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A three- site interlaboratory reproducibility evaluation of the Etest(R) concentration gradient strip method for testing antifungal susceptibilities was conducted using 30 strains of dermatophytes exposed to strips loaded with ketoconazole ( KTZ), itraconazole ( ITZ), amphotericin B ( AMB) and fluconazole ( FCZ). Etest(R) minimal inhibitory concentrations were compared with those obtained using a broth microdilution method. All isolates produced clearly detectable growth at 28 degreesC within 72 - 96 h for reading with the Etest(R) method. The highest interlaboratory agreement between Etest(R) and the microdilution method was shown with FCZ ( 94%), and the lowest was seen with KTZ ( 60%). Overall, agreement between the Etest(R) and microdilution method was variable. It was excellent for AMB ( 97%), good for ITZ ( 80%) and KTZ ( 77%), and low for fluconazole ( 27%).

引用

页码：125 / 130

页数：6