Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

被引：48

作者：

Cha, Hyunjung ^{[1
]}

Kim, Hyeran ^{[1
]}

Joung, Younju ^{[2
]}

Kang, Hyunju ^{[1
]}

Moon, Jeong ^{[1
,3
]}

Jang, Hyowon ^{[1
]}

Park, Sohyun ^{[2
]}

Kwon, Hyung-Jun ^{[4
]}

Lee, In-Chul ^{[4
]}

Kim, Sunjoo ^{[5
]}

Yong, Dongeun ^{[6
,7
]}

Yoon, Sun-Woo ^{[1
]}

Park, Sung-Gyu ^{[8
]}

Guk, Kyeonghye ^{[1
]}

Lim, Eun-Kyung ^{[1
,9
]}

Park, Hyun Gyu ^{[3
]}

Choo, Jaebum ^{[2
]}

Jung, Juyeon ^{[1
]}

Kang, Taejoon ^{[1
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol KRIBB, Bionanotechnol Res Ctr, Daejeon 34141, South Korea

[2] Chung Ang Univ, Dept Chem, Seoul 06974, South Korea

[3] Korea Adv Inst Sci & Technol KAIST, Dept Chem & Biomol Engn, Daejeon 34141, South Korea

[4] Korea Res Inst Biosci & Biotechnol KRIBB, Funct Biomat Res Ctr, Jeongeup 56212, South Korea

[5] Gyeongsang Natl Univ, Dept Lab Med, Coll Med, Jinju 52828, South Korea

[6] Yonsei Univ, Res Inst Bacterial Resistance, Coll Med, Seoul 03722, South Korea

[7] Yonsei Univ, Dept Lab Med, Coll Med, Seoul 03722, South Korea

[8] Korea Inst Mat Sci KIMS, Nano Bio Convergence Dept, Chang Won 51508, South Korea

[9] Univ Sci & Technol UST, KRIBB Sch Biotechnol, Dept Nanobiotechnol, Daejeon 34113, South Korea

来源：

BIOSENSORS & BIOELECTRONICS | 2022年 / 202卷

基金：

新加坡国家研究基金会;

关键词：

Antibody; Coronavirus disease 2019; Immunoassay; Nanoparticle; Surface-enhanced Raman scattering; Severe acute respiratory syndrome coronavirus 2; SARS-COV-2;

D O I：

10.1016/j.bios.2022.114008

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.

引用

页数：9

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