Phylogenetic analysis of the DNA sequence of the non-coding region of nuclear ribosomal DNA and chloroplast of Ephedra plants in China

被引：40

作者：

Long, C

Kakiuchi, N

Takahashi, A

Komatsu, K

Cai, S

Mikage, M

机构：

[1] Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 9200934, Japan

[2] Museum Nat & Human Activ, Sanda, Hyogo, Japan

[3] Toyama Med & Pharmaceut Univ, Inst Natl Med, Toyama, Japan

[4] Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100871, Peoples R China

来源：

PLANTA MEDICA | 2004年 / 70卷 / 11期

关键词：

Ephedra; Ephedraceae; nuclear ribosomal DNA; internal tran-;

D O I：

10.1055/s-2004-832651

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Twenty-four Ephedra plants belonging to 8 species grown in the northern and western parts of China were phylogenetically analyzed for their non-coding DNA sequences, internal transcribed spacers (ITSs) of nuclear ribosomal DNA as well as trnL intron and intergenic spacers between trnL and trnF (trnL/trnF) of the chloroplast. Based on the ITS sequences, the 8 species could be divided into 3 groups: Group 1 (Ephedra intermedia, E. sinica, E. przewalskii), Group 2 (E. equisetina, E. monosperma, E. gerardiana), and Group 3 (E. likiangensis, E. minuta). The species classified into Group 1 grow mainly in the north, Group 3 in the south and Group 2 in the center, suggesting their genetic and geographic relationships. A specific primer set was designed to classify the 3 groups by routine PCR. Combined analysis of ITS and trnL/trnF differentiated the 8 Ephedra species.

引用

页码：1080 / 1084

页数：5