Increased mRNA expression of phospholipase D (PLD) isozymes during granulocytic differentiation of HL60 cells

被引：31
作者：
Nakashima, S
Ohguchi, K
Frohman, MA
Nozawa, Y
机构：
[1] SUNY Stony Brook, Dept Pharmacol Sci, Stony Brook, NY 11794 USA
[2] SUNY Stony Brook, Inst Mol & Cell Biol, Stony Brook, NY 11794 USA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1998年 / 1389卷 / 03期
关键词：
phospholipase D; HL60; cell; granulocytic differentiation;
D O I：
10.1016/S0005-2760(97)00153-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In response to dibutyryl cyclic AMP (dbcAMP) and all-trans retinoic acid (ATRA), HL60 cells differentiate into granulocyte-like cells. Membrane-associated phospholipase D (PLD) activity in response to guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) or phorbol myristate acetate (PMA) was upregulated by these treatments. Reverse transcriptase-polymerase chain reaction (RT-PCR) analyses revealed that both hPLD1a and hPLD1b mRNAs were expressed in HL60 cells and that their expression levels increased during differentiation. hPLD2 mRNA levels rose dramatically during differentiation. These results suggest that the PLD genes undergo changes in transcriptional regulation during granulocytic differentiation of HL60 cells. (C) 1998 Elsevier Science B.V.
引用
收藏
页码：173 / 177
页数：5
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