Enhanced Detection of Enteroviruses in Clinical Samples by Reverse Transcription-PCR Using Complementary Locked Primer Technology

被引：8

作者：

Hong, JiYoung ^{[1
]}

Kang, Byunghak ^{[1
]}

Kim, Ahyoun ^{[1
]}

Hwang, Seoyoun ^{[1
]}

Lee, Sunwha ^{[1
]}

Kim, Jonghyen ^{[2
]}

Lee, Hyun-Young ^{[3
]}

Kang, Sang-Hyeon ^{[3
]}

Cheon, Doo-Sung ^{[1
]}

机构：

[1] Korea Ctr Dis Control & Prevent, Ctr Infect Dis, Natl Inst Hlth, Div Enter & Hepatitis Viruses, Seoul 122701, South Korea

[2] Catholic Univ, Coll Med, Dept Pediat, Suwon, South Korea

[3] INtRON Biotechnol Inc, Songnam 462120, Gyeonggi Do, South Korea

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 02期

关键词：

POLYMERASE-CHAIN-REACTION; CEREBROSPINAL-FLUID SPECIMENS; CENTRAL-NERVOUS-SYSTEM; RAPID DETECTION; PATIENT-MANAGEMENT; ASEPTIC-MENINGITIS; DIAGNOSIS; ASSAY; INFECTIONS; UTILITY;

D O I：

10.1128/JCM.01790-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.

引用

页码：615 / 616

页数：2

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