Comparison of coherent and spontaneous Raman microspectroscopies for noninvasive detection of single bacterial endospores

被引:130
作者
Petrov, Georgi I.
Arora, Rajan
Yakovlev, Vladislav V.
Wang, Xi
Sokolov, Alexei V.
Scully, Marlan O.
机构
[1] Univ Wisconsin, Dept Phys, Milwaukee, WI 53201 USA
[2] Texas A&M Univ, Inst Quantum Studies, College Stn, TX 77843 USA
[3] Texas A&M Univ, Dept Phys, College Stn, TX 77843 USA
[4] Princeton Univ, Appl Phys & Mat Sci Grp, Princeton, NJ 08544 USA
关键词
microscopy; nonlinear optics; scattering stimulated; ultrafast optics;
D O I
10.1073/pnas.0702107104
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Single bacterial spores were analyzed by using nonlinear Raman microspectroscopy based on coherent anti-Stokes Raman scattering (CARS). The Raman spectra were retrieved from CARS spectra and found to be in excellent agreement with conventionally collected Raman spectra. The phase retrieval method based on maximum entropy model revealed significant robustness to external noise. The direct comparison of signal amplitudes exhibited a factor of 100 stronger CARS signal, as compared with the Raman signal.
引用
收藏
页码:7776 / 7779
页数:4
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