Determination of bile acids in pharmaceutical formulations using micellar electrokinetic chromatography

被引：21
作者：
Rodríguez, VG [1 ]
Lucangioli, SE [1 ]
Otero, GCF [1 ]
Carducci, CN [1 ]
机构：
[1] Univ Buenos Aires, Fac Pharm & Biochem, Dept Analyt & Phys Chem, RA-1113 Buenos Aires, DF, Argentina
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2000年 / 23卷 / 2-3期
关键词：
micellar electrokinetic chromatography; bile acids; pharmaceuticals;
D O I：
10.1016/S0731-7085(00)00314-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A micellar electrokinetic chromatography method (MEKC) has been developed and validated for the determination of bile acids (BA) such as ursodeoxycholic acid (UDCA), dehydrocholic acid (DHCA) and deoxycholic acid (DCA) in pharmaceuticals for quality control purpose. The background electrolyte consisted of 20 mM borate-phosphate buffer containing 50 mM sodium dodecylsulfate (SDS), and acetonitrile as additive. UV detection was set at 185 nm. Selectivity, linearity, range, repeatability, intermediate precision and accuracy showed good results. Comparison of the values obtained by MEKC and HPLC methods were in close agreement. (C) 2000 Elsevier Science B.V. All rights reserved.
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收藏
页码：375 / 381
页数：7
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