Hyperexcitability and changes in activities of Ca2+/calmodulin-dependent kinase II and mitogen-activated protein kinase in the hippocampus of rats exposed to 1-bromopropane

被引:16
|
作者
Fueta, Y
Fukunaga, K
Ishidao, T
Hori, H
机构
[1] Univ Occupat & Environm Hlth, Sch Hlth Sci, Dept Med Technol 1, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan
[2] Kumamoto Univ, Sch Med, Dept Pharmacol, Kumamoto 8600811, Japan
[3] Univ Occupat & Environm Hlth, Sch Hlth Sci, Dept Environm Management 1, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan
关键词
hyperexcitability; CaMKIII; MAPK; inhalation; 1-bromopropane; rat hippocampus;
D O I
10.1016/S0024-3205(02)02247-6
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Chronic inhalation of 1-bromopropane (1-BP), a substitute of ozone-depleting chlorofluorocarbons, has been suspected of having central neurotoxicity (Clinical Neurology and Neurosurgery 101 (1999) 199; Journal of Occupational Health 44 (2002) 1) for humans. In animal experiments, 1-BP inhalation (1500 ppm) caused hyperexcitability in the CA1 and the dentate gyrus (DG) [Journal of Occupational Health 42 (2000) 149, Journal of Occupational Health 44(2002)156]. We studied whether the hyperexcitability is associated with changes of Ca2+/ calmodulin-dependent kinase II (CaMKII), mitogen-activated protein kinase (MAPK), and protein kinase C (PKC). Male Wistar rats were exposed to 1-BP for 6 hours in a day in an exposure chamber with a concentration of 700 ppm for 8 weeks. After the inhalation, paired-pulse ratios of field excitatory postsynaptic potentials and population spikes (PSs) were analyzed in the CA1 and DG of hippocampal slices. Control rats were then given fresh air in the inhalation chamber. Semiquantitative immunoblotting analyses of protein kinases using antibodies against active and conventional protein kinases were done using the whole hippocampus. A paired-pulse ratio of PS was increased at the 5 ins interpulse interval in the CA1 and at the 10-20 ms interpulse intervals in the DG. The amount of active MAPK and total amount of CaMKIIalpha and beta were significantly increased by 28, 29, and 46% compared to control, respectively, without any change in PKC activity. In contrast, the amount of active CaMKIIbeta was decreased to 78%. These results suggest that modifications of intracellular signaling cascades are associated with hyperexcitability that occurred in the hippocampal formation of rats exposed to the chronic inhalation of 1-BP. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:521 / 529
页数:9
相关论文
共 50 条
  • [21] CA2+/CALMODULIN-DEPENDENT PROTEIN-KINASE KINASE CASCADE
    SUGITA, R
    MOCHIZUKI, H
    ITO, T
    YOKOKURA, H
    KOBAYASHI, R
    HIDAKA, H
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 203 (01) : 694 - 701
  • [22] Regulatory mechanism of Ca2+/calmodulin-dependent protein kinase kinase
    Tokumitsu, H
    Muramatsu, M
    Ikura, M
    Kobayashi, R
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (26) : 20090 - 20095
  • [23] Molecular Cloning of Ca2+/Calmodulin-Dependent Protein Kinase Kinase a
    Kitani, T.
    Okuno, S.
    Fujisawa, H.
    Journal of Biochemistry, 122 (01):
  • [24] The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases
    Hurley, RL
    Anderson, KA
    Franzone, JM
    Kemp, BE
    Means, AR
    Witters, LA
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (32) : 29060 - 29066
  • [25] Regulation of mitogen-activated protein kinases by a calcium/calmodulin-dependent protein kinase cascade
    Enslen, H
    Tokumitsu, H
    Stork, PJS
    Davis, RJ
    Soderling, TR
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (20) : 10803 - 10808
  • [26] Regulation of multifunctional Ca2+/calmodulin-dependent protein kinases by Ca2+/calmodulin-dependent protein kinase phosphatase
    Ishida, A
    Okuno, S
    Kitani, T
    Kameshita, I
    Fujisawa, H
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 253 (01) : 159 - 163
  • [27] Tyrosine kinase activity of a Ca2+/calmodulin-dependent protein kinase II catalytic fragment
    Sugiyama, Yasunori
    Ishida, Atsuhiko
    Sueyoshi, Noriyuki
    Kameshita, Isamu
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 377 (02) : 648 - 652
  • [28] Role of protein kinase C, Ca2+/calmodulin-dependent protein kinase II, and mitogen-activated protein kinases in volatile anesthetic-induced relaxation in newborn rabbit pulmonary artery
    Su, JY
    Anhkiet, CV
    ANESTHESIOLOGY, 2003, 99 (01) : 131 - 137
  • [29] Contribution of kinase activity of Ca2+/calmodulin-dependent protein kinase IIα to hippocampus- and amygdala-dependent memory formation
    Yamagata, Yoko
    Yanagawa, Yuchio
    Imoto, Keiji
    NEUROSCIENCE RESEARCH, 2011, 71 : E181 - E181
  • [30] Phosphorylation of calmodulin by Ca2+/calmodulin-dependent protein kinase IV
    Ishida, A
    Kameshita, I
    Okuno, S
    Kitani, T
    Fujisawa, H
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2002, 407 (01) : 72 - 82