Unique structure and function of viral rhodopsins

被引：37

作者：

Bratanov, Dmitry ^{[1
,2
,3
]}

Kovalev, Kirill ^{[1
,2
,3
,4
,5
,6
]}

Machtens, Jan-Philipp ^{[6
,7
]}

Astashkin, Roman ^{[3
,4
]}

Chizhov, Igor ^{[8
]}

Soloviov, Dmytro ^{[4
,9
,10
,11
]}

Volkov, Dmytro ^{[1
,2
,4
]}

Polovinkin, Vitaly ^{[1
,3
]}

Zabelskii, Dmitrii ^{[1
,2
,4
]}

Mager, Thomas ^{[12
]}

Gushchin, Ivan ^{[4
]}

Rokitskaya, Tatyana ^{[13
]}

Antonenko, Yuri ^{[13
]}

Alekseev, Alexey ^{[1
,2
,4
,5
]}

Shevchenko, Vitaly ^{[1
,2
,4
,5
]}

Yutin, Natalya ^{[14
]}

Rosselli, Riccardo ^{[15
]}

Baeken, Christian ^{[1
,2
]}

Borshchevskiy, Valentin ^{[1
,2
,4
]}

Bourenkov, Gleb ^{[16
]}

Popov, Alexander ^{[17
]}

Balandin, Taras ^{[1
,2
]}

Buldt, Georg ^{[4
]}

Manstein, Dietmar J. ^{[8
]}

Rodriguez-Valera, Francisco ^{[15
]}

Fahlke, Christoph ^{[4
,6
]}

Bamberg, Ernst ^{[4
,12
]}

Koonin, Eugene ^{[14
]}

Gordeliy, Valentin ^{[1
,2
,3
,4
]}

机构：

[1] Forschungszentrum Julich, ICS 6 Struct Biochem, ICS, Julich, Germany

[2] Forschungszentrum Julich, JuStruct Julich Ctr Struct Biol, Julich, Germany

[3] Univ Grenoble Alpes, Inst Biol Struct JP Ebel, CEA, CNRS, Grenoble, France

[4] Moscow Inst Phys & Technol, Dolgoprudnyi, Russia

[5] Univ Aachen RWTH, Inst Crystallog, Aachen, Germany

[6] Forschungszentrum Julich, ICS 4 Zellulare Biophys, ICS, Julich, Germany

[7] Univ Aachen RWTH, Dept Mol Pharmacol, Aachen, Germany

[8] Hannover Med Sch, Inst Biophys Chem, Hannover, Germany

[9] Joint Inst Nucl Res, Dubna, Russia

[10] Taras Shevchenko Natl Univ Kyiv, Kiev, Ukraine

[11] NAS Ukraine, Inst Safety Problems Nucl Power Plants, Kiev, Ukraine

[12] Max Planck Inst Biophys, Frankfurt, Germany

[13] Lomonosov Moscow State Univ, Belozersky Inst Physicochem Biol, Moscow, Russia

[14] NIH, Natl Ctr Biotechnol Informat, NLM, Bldg 10, Bethesda, MD 20892 USA

[15] Univ Miguel Hernandez, Dept Prod Vegetal & Microbiol, Evolutionary Genom Grp, Alacant, Spain

[16] DESY, European Mol Biol Lab, Hamburg Unit, Hamburg, Germany

[17] European Synchrotron Radiat Facil, Grenoble, France

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

俄罗斯基础研究基金会; 美国国家卫生研究院; 俄罗斯科学基金会;

关键词：

ION-CHANNEL; MOLECULAR-DYNAMICS; MEMBRANE-PROTEINS; HYDROPHOBIC GATE; PURPLE MEMBRANE; PROTON PUMP; WEB SERVER; BACTERIORHODOPSIN; PHOTOCYCLE; BILAYER;

D O I：

10.1038/s41467-019-12718-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recently, two groups of rhodopsin genes were identified in large double-stranded DNA viruses. The structure and function of viral rhodopsins are unknown. We present functional characterization and high-resolution structure of an Organic Lake Phycodnavirus rhodopsin II (OLPVRII) of group 2. It forms a pentamer, with a symmetrical, bottle-like central channel with the narrow vestibule in the cytoplasmic part covered by a ring of 5 arginines, whereas 5 phenylalanines form a hydrophobic barrier in its exit. The proton donor E42 is placed in the helix B. The structure is unique among the known rhodopsins. Structural and functional data and molecular dynamics suggest that OLPVRII might be a light-gated pentameric ion channel analogous to pentameric ligand-gated ion channels, however, future patch clamp experiments should prove this directly. The data shed light on a fundamentally distinct branch of rhodopsins and may contribute to the understanding of virus-host interactions in ecologically important marine protists.

引用

页数：13

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