Purification and characterization of antioxidant peptides from enzymatically hydrolyzed ark shell (Scapharca subcrenata)

被引：40
作者：
Jin, Ji-Eun [1 ]
Ahn, Chang-Bum [2 ]
Je, Jae-Young [1 ]
机构：
[1] Pukyong Natl Univ, Dept Marine Bio Convergence Sci, Busan 48547, South Korea
[2] Chonnam Natl Univ, Div Food & Nutr, Gwangju 61186, South Korea
来源：
PROCESS BIOCHEMISTRY | 2018年 / 72卷
关键词：
Ark shell; Enzymatic hydrolysis; Antioxidant peptides; Purification; Antioxidant activities; MESENCHYMAL STEM-CELLS; PROTEIN HYDROLYSATE; BIOACTIVE PEPTIDES; OXIDATIVE STRESS; LDL OXIDATION; IDENTIFICATION;
D O I：
10.1016/j.procbio.2018.06.001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Information of bioactive peptides derived from ark shell is seldom reported. In this study, ark shell was hydrolyzed by pepsin with enzyme to substrate (E/S) ratios of 1:100 and 1:500 for 1-4 h. Ark shell hydrolysates (ASHs) were subjected to evaluate their antioxidant activities by free radical scavenging and oxygen radical absorbance capacity (ORAC) assays. Antioxidant peptides (P) were purified and identified as MCLDSCLL (P1) and HPLDSLCL (P2). P1 and P2 showed potent antioxidant activities with IC(50 )value of 19.47 +/- 0.35 and 74.36 +/- 0.54 mu M, and glutathione (GSH), as positive control, showed 19.31 +/- 0.36 mu M against DPPH radical. P1 and P2 exhibited 678.36 +/- 5.33 mu M trolox equivalent (TE)/mM P1 and 778.86 +/- 9.64 mu M TE/mM P2 against ABTS(+) radical scavenging, and ORAC values of 333.91 +/- 26.05 mu M TE/mM P1 and 209.81 +/- 21.74 mu M TE/mM P2. P1 and P2 also showed strong reducing power. In addition, P2 strongly inhibited copper-catalyzed human LDL oxidation.
引用
收藏
页码：170 / 176
页数：7
相关论文
共 29 条
[1]   Purification and antioxidant properties of octapeptide from salmon byproduct protein hydrolysate by gastrointestinal digestion [J].
Ahn, Chang-Bum ;
Kim, Jeong-Gyun ;
Je, Jae-Young .
FOOD CHEMISTRY, 2014, 147 :78-83
[2]   Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate [J].
Aleman, A. ;
Gimenez, B. ;
Perez-Santin, E. ;
Gomez-Guillen, M. C. ;
Montero, P. .
FOOD CHEMISTRY, 2011, 125 (02) :334-341
[3]   Activity of myricetin and other plant-derived polyhydroxyl compounds in human LDL and human vascular endothelial cells against oxidative stress [J].
Bertin, Riccardo ;
Chen, Zheng ;
Marin, Raffaella ;
Donati, Maddalena ;
Feltrinelli, Angela ;
Montopoli, Monica ;
Zambon, Sabina ;
Manzato, Enzo ;
Froldi, Guglielmina .
BIOMEDICINE & PHARMACOTHERAPY, 2016, 82 :472-478
[4]   Oxidative Stress and Antioxidant Defense [J].
Birben, Esra ;
Sahiner, Umit Murat ;
Sackesen, Cansin ;
Erzurum, Serpil ;
Kalayci, Omer .
WORLD ALLERGY ORGANIZATION JOURNAL, 2012, 5 :9-19
[5]   Purification and identification of novel antioxidant peptides from enzymatic hydrolysates of sardinelle (Sardinella aurita) by-products proteins [J].
Bougatef, Ali ;
Nedjar-Arroume, Naima ;
Manni, Laila ;
Ravallec, Rozenn ;
Barkia, Ahmed ;
Guillochon, Didier ;
Nasri, Moncef .
FOOD CHEMISTRY, 2010, 118 (03) :559-565
[6]   Hibiscus anthocyanins-rich extract inhibited LDL oxidation and oxLDL-mediated macrophages apoptosis [J].
Chang, Yun-Ching ;
Huang, Kai-Xun ;
Huang, An-Chung ;
Ho, Yung-Chyuan ;
Wang, Chau-Jong .
FOOD AND CHEMICAL TOXICOLOGY, 2006, 44 (07) :1015-1023
[7]   Anti-atherosclerotic potential of gossypetin via inhibiting LDL oxidation and foam cell formation [J].
Chen, Jing-Hsien ;
Tsai, Chia-Wen ;
Wang, Chi-Ping ;
Lin, Hui-Hsuan .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2013, 272 (02) :313-324
[8]   Isolation and characterization of three antioxidant peptides from protein hydrolysate of bluefin leatherjacket (Navodon septentrionalis) heads [J].
Chi, Chang-Feng ;
Wang, Bin ;
Wang, Yu-Mei ;
Zhang, Bin ;
Deng, Shang-Gui .
JOURNAL OF FUNCTIONAL FOODS, 2015, 12 :1-10
[9]   Free radicals, antioxidants, and nutrition [J].
Fang, YZ ;
Yang, S ;
Wu, GY .
NUTRITION, 2002, 18 (10) :872-879
[10]   Potential uses of caseinophosphopeptides [J].
FitzGerald, RJ .
INTERNATIONAL DAIRY JOURNAL, 1998, 8 (5-6) :451-457
123