Multitoxin biosensor-mass spectrometry analysis: a new approach for rapid, real-time, sensitive analysis of staphylococcal toxins in food

被引:86
作者
Nedelkov, D
Rasooly, A
Nelson, RW
机构
[1] Intrins Bioprobes Inc, Tempe, AZ 85281 USA
[2] US FDA, Div Microbiol Studies, OSRS, CFSAN, Washington, DC 20204 USA
关键词
biomolecular interaction analysis mass spectrometry; surface plasmon resonance; MALDI-TOF mass spectrometry; enterotoxin B; toxic-shock syndrome toxin-1; Staphylococcus aureus;
D O I
10.1016/S0168-1605(00)00328-7
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Biomolecular interaction analysis mass spectrometry (BIA-MS) was applied to detection of bacterial toxins in food samples. This two-step approach utilizes surface plasmon resonance (SPR) to detect the binding of the toxin(s) to antibodies immobilized on a surface of a sensor chip. SPR detection is then followed by identification of the bound toxin(s) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Staphylococcal enterotoxin B (SEB) was readily detected in milk and mushroom samples at levels of 1 ng/ml. In addition, non-specific binding of food components to the immobilized antibody and to the sensor chip surface was detected. To evaluate the applicability of BIA-MS in the analysis of materials containing multiple toxic components, sample containing both SEE and toxic-shock syndrome toxin-l was analyzed. Both toxins were successfully and simultaneously detected through the utilization of multiaffinity sensor chip surfaces. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:1 / 13
页数:13
相关论文
共 53 条
[1]  
ARCHER D L, 1988, Clinical Microbiology Reviews, V1, P377
[2]  
Bean N H, 1990, MMWR CDC Surveill Summ, V39, P15
[3]  
Beavis R C, 1989, Rapid Commun Mass Spectrom, V3, P436, DOI 10.1002/rcm.1290031208
[4]  
Bergdoll MS, 1996, ADV EXP MED BIOL, V391, P465
[5]  
BERGDOLL MS, 1981, LANCET, V1, P1017
[6]  
BETLEY MJ, 1984, P NATL ACAD SCI-BIOL, V81, P5179, DOI 10.1073/pnas.81.16.5179
[7]  
BETLEY MJ, 1986, ANNU REV MICROBIOL, V40, P577, DOI 10.1146/annurev.mi.40.100186.003045
[8]  
Bunning V. Kelly, 1997, World Health Statistics Quarterly, V50, P51
[9]   ANALYSIS OF THE MITOGENIC EFFECTS OF TOXIC SHOCK TOXIN ON HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS INVITRO [J].
CALVANO, SE ;
QUIMBY, FW ;
ANTONACCI, AC ;
REISER, RF ;
BERGDOLL, MS ;
DINEEN, P .
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1984, 33 (01) :99-110
[10]   INTERACTION OF STAPHYLOCOCCUS-AUREUS TOXIN SUPERANTIGENS WITH HUMAN T-CELLS [J].
CHOI, YW ;
KOTZIN, B ;
HERRON, L ;
CALLAHAN, J ;
MARRACK, P ;
KAPPLER, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (22) :8941-8945